Brilliant Violet 421 anti-mouse TER-119/Erythroid Cells Antibody anti-TER-119

Pricing & Availability

Clone: TER-119 (See other available formats)
Regulatory Status: RUO
Other Names: Ly-76
Isotype: Rat IgG2b, κ
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Product Citations: publications

TER-119_BV421_1_051311 — C57BL/6 splenocytes mixed with red blood cells were stained with CD45 FITC and TER-119 Brilliant Violet 421™ (top) or rat IgG2b Brilliant Violet 421™ isotype control (bottom).

Compare all formats See Brilliant Violet 421™ spectral data

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116233

125 µL

155€

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116234

50 µg

219€

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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Day-14 fetal liver cells from a C57BL/6 mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting¹, complement-mediated cytotoxicity³, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
Vannucchi AM, et al. 2000. Blood 95:2559.
Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
Grisendi S, et al. 2005. Nature 437:147. (FC)
Bourdeau A, et al. 2007. Blood 109:4220.
Chappaz S, et al. 2007. Blood 110:3862. (FC)
Heuser M, et al. 2007. Blood 110:1639. (FC)
Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed

Product Citations

Cui Z, et al. 2022. Bone Res. 10:58. PubMed
Romera–Hernández M, et al. 2020. Cell Reports. 30(1):37-45.e3.. PubMed
Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
Chuang CH, et al. 2020. Cancer Research. 81:567. PubMed
Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
Ma Z, et al. 2022. Methods Mol Biol. 2471:49. PubMed
Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
Ombrato L, et al. 2021. Nat Protoc. 16:872. PubMed
Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
Jolly A, et al. 2022. Cell Rep Methods. 2:100315. PubMed
Nagatake T, et al. 2021. Int Immunol. 33:171. PubMed
Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
Hu B, et al. 2020. J Clin Invest. 130:3483. PubMed
Peltzer N, et al. 2018. Nature. 557:112. PubMed
Mahmoudi S, et al. 2019. Nature. 574:553. PubMed

RRID

AB_10933426 (BioLegend Cat. No. 116233)
AB_2562917 (BioLegend Cat. No. 116234)

Antigen Details

Structure: Associated with glycophorin A, 52 kD
Distribution: Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E
Cell Type: Erythrocytes
Biology Area: Immunology
Antigen References: 1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.
Gene ID: 104231 View all products for this Gene ID
UniProt: View information about TER-119 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All TER-119 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse TER-119/Erythroid Cells	TER-119	FC
Biotin anti-mouse TER-119/Erythroid Cells	TER-119	FC
FITC anti-mouse TER-119/Erythroid Cells	TER-119	FC
PE anti-mouse TER-119/Erythroid Cells	TER-119	FC
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Purified anti-mouse TER-119/Erythroid Cells	TER-119	FC,IHC-F,IHC-P,IP,WB
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells	TER-119	FC
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells	TER-119	FC
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells	TER-119	FC
PerCP anti-mouse TER-119/Erythroid Cells	TER-119	FC
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Pacific Blue™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready)	TER-119	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells	TER-119	PG
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells	TER-119	FC
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells	TER-119	PG
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells	TER-119	PG
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells	TER-119	FC,IHC-F,IHC-P,IP,WB,CMCD
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells	TER-119	FC
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody	TER-119	FC

Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 12/04/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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