Biotinylated Recombinant Human B7-2/CD86-Fc Chimera (carrier-free)

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Other Names
CD86, B7-2, T lymphocyte activation antigen CD86, Activation B7-2 antigen, CD28 antigen ligand 2, CD28LG2B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1
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Human_B7-2slashCD86-Fc_Chumera_RECOM_CF_1_020119.png
When human CTLA-4 (Cat. No. 591902) is immobilized at 1.0 µg/mL, Human B7-2/CD86-Fc Chimera Biotinylated Protein binds with EC50 of 8 - 32 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Human_B7-2slashCD86-Fc_Chumera_RECOM_CF_1_020119.png
    When human CTLA-4 (Cat. No. 591902) is immobilized at 1.0 µg/mL, Human B7-2/CD86-Fc Chimera Biotinylated Protein binds with EC50 of 8 - 32 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Human_B7-2slashCD86-Fc_Chumera_RECOM_CF_2_020119.png
    Stability testing for Human B7-2/CD86-Fc Chimera Biotinylated Protein. Human B7-2/CD86-Fc Chimera Biotinylated Protein was aliquoted in PBS, pH 7.2, 5% Glycerol at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested for their ability to bind immobilized human CTLA-4 (Cat. No. 591902).
Cat # Size Price Quantity Avail. Save
779402 10 µg 100€
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779404 25 µg 196€
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779406 100 µg 584€
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Description

CD86, also known as B7-2, is a member of the cell surface immunoglobulin superfamily.  B7 family members are transmembrane molecules that exist predominantly as monomers. They are classified into three groups according to their functions. Group I members include B7-1, B7-2, and B7-H2.  These members are either co-stimulatory or co-inhibitory in modulating T cell response.  Group II members include B7-H1 (PD-L1) and B7-DC (PD-L2) which are ligands for PD-1.  These members are involved in regulating induction and maintenance of peripheral immune tolerance.  Group III members include B7-H3 and B7-H4 whose role in T cell regulation has yet to be defined.  CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4).  CD86 is expressed earlier in the immune response than CD80.  CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human B7-2/CD86, amino acid Leu20-His245 (accession # P42081) with a C-terminal human IgG1 (Pro100-Lys330) and 6x His tag expressed in 293E cells.
Molecular Mass
The unlabeled 465 amino acid recombinant protein has a predicted molecular mass of approximately 52.9 kD. The DTT-reduced protein migrates at approximately 70 kD and non-reduced protein migrates at approximately 140 kD by SDS-PAGE. The predicted N-terminal amino acid is Leu.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 μm filtered protein solution is in PBS, pH7.2, 5% Glycerol
Endotoxin Level
Less than 0.1 EU per μg protein as determined by the LAL method.
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg size is lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
When human CTLA4a-Fc is immobilized at 1.0 μg/mL (100 μL/ well), biotinylated human CD86-Fc Chimera binds with an EC50 of 8 - 32 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Disulfide-linked homodimer, biotinylated via amines
Distribution

Activated antigen presenting cells, including B cells, dendritic cells and macrophages

Function
B7-2 is a costimulatory molecule that is required for T cell proliferation and IL-2 production.
Interaction
Leukocytes, endothelial cells
Ligand/Receptor
CD28, CTLA4
Bioactivity
Measured by its ability to bind human CTLA4-Fc Chimera
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Soluble Receptors
Antigen References
  1. Freeman GJ, et al. 1993. Science 262:909.
  2. Chen C, et al. 1994. J. Immunol. 152:4929.
  3. Lanier LL, et al. 1995. J. Immunol. 154:97.
  4. Carreno BM, Collins M. 2002. Annu. Rev. Immunol. 20:29.
  5. Rudd CE, et al. 2009. Immunol. Rev. 229:12.
  6. Wing K, et al. 2011. Trends Immunol. 32:428.
Gene ID
942 View all products for this Gene ID
UniProt
View information about B7-2 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 02/01/2019

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