- Clone
- 55R-170 (See other available formats)
- Other Names
- TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- 1 reviews
- Product Citations
- publications
-
NIH/3T3 cell line stained with biotinylated 55R-170, followed by Sav-PE
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 112903 | 50 µg | 60€ | ||||
| 112904 | 500 µg | 184€ | ||||
CD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. The 55R-170 antibody has been shown to block in vitro and in vivo receptor signaling initiated by ligand binding.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- E. coli-expressed extracellular domain of the mouse TNFRI protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
ELISA Detection - Reported in the literature - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Flow Cytometry: For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112904) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).
ELISA Detection1: The biotinylated 55R-170 antibody is useful as a detection antibody when paired with the Purified 55R-286 antibody (Cat. No. 113002) as the capture antibody.
Additional reported applications (for the relevant formats) include:immunoprecipitation1, and In vitro and In vivo receptor blocking1. The Ultra-LEAF™Purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 112905 & 112906). - Application References
-
- Sheehan, K. C., et al. 1995. J. Exp. Med. 181:607.
- RRID
-
AB_313528 (BioLegend Cat. No. 112903)
AB_313529 (BioLegend Cat. No. 112904)
Antigen Details
- Structure
- TNFR superfamily, 55 kD
- Distribution
-
Variety of cell types at low levels
- Function
- Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
- Ligand/Receptor
- TNF-α, LT-α (TNF-β)
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Aggarwal, B. B., et al. 1985. Nature 318 665.
2. Chan, F. K.M., et al. 2000. Science 288:2351.
3. Loetscher, H., et al. 1990. Cell 61:351.
4. Rothe, J., et al. 1993. Nature 364:798. - Gene ID
- 21937 View all products for this Gene ID
- UniProt
- View information about CD120a on UniProt.org
Reviews
-
- Title
- Rating
- Date
-
12/09/2016
Related Pages & Pathways
Pathways
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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