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Biotin anti-human CD5 Antibody

Pricing & Availability
Clone
UCHT2 (See other available formats)
Regulatory Status
RUO
Workshop
III 518
Other Names
Leu-1, Ly-1, T1, Tp67
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
UCHT2_Biotin_060607
Human peripheral blood lymphocytes were stained with biotinylated CD5 (clone UCHT2) (filled histogram) or biotinylated mouse IgG1, κ isotype control (open histogram).
  • UCHT2_Biotin_060607
    Human peripheral blood lymphocytes were stained with biotinylated CD5 (clone UCHT2) (filled histogram) or biotinylated mouse IgG1, κ isotype control (open histogram).
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300604 100 µg 151€
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Description

CD5 is a 67 kD single chain type I glycoprotein also known as Leu-1, Ly-1 and T1. It is a member of the scavenger receptor superfamily found on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-CLL), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions upon binding to ligands such as CD72.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Capuchin Monkey, Common Marmoset, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, it is recommended to use at ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting2 and immunohistochemical staining of acetone-fixed frozen sections2,5.

Application References
  1. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
  2. Renaudineau Y, et al. 2005. Blood 106:2781. (WB IHC)
  3. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437.
  4. Saliba AE, et al. 2010. P. Natl. Acad. Sci. USA 107:14524. PubMed
  5. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
RRID
AB_314090 (BioLegend Cat. No. 300604)

Antigen Details

Structure
Scavenger receptor superfamily, 67 kD
Distribution

T cells, thymocytes, B cell subset, B cell CLL, peripheral blood dendritic cells

Function
Modulates TCR, BCR signaling, thymocyte maturation, T-B cell interaction
Ligand/Receptor
CD72
Cell Type
B cells, Dendritic cells, Leukemia, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Kipps T. 1988. Adv. Immunol. 47:117.
2. Resnick D, et al. 1993. Trends Biochem. Sci. 19:5.
3. Wood GS, et al. 1992. Am. J. Pathol. 14:789.

Gene ID
921 View all products for this Gene ID
UniProt
View information about CD5 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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