Biotin anti-6-His Epitope Tag Antibody (Previously Covance catalog# BIOT-156L)

Pricing & Availability
6-His (See other available formats)
Regulatory Status
Other Names
polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Covance Catalog# BIOT-156L
Mouse IgG1, κ
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Product Citations
Western blot analysis of 0.5 µl Posi-tag (Cat. No. 931301) using Biotin anti 6-His Mouse primary antibody (Clone 6-His, 1:2000 dilution, 0.5 µg/ml) and HRP Avidin secondary antibody (Cat. No. 405103, 1:1000 dilution).
  • 6-His_EpitopeTag_1_011918
    Western blot analysis of 0.5 µl Posi-tag (Cat. No. 931301) using Biotin anti 6-His Mouse primary antibody (Clone 6-His, 1:2000 dilution, 0.5 µg/ml) and HRP Avidin secondary antibody (Cat. No. 405103, 1:1000 dilution).
  • 6-His_EpitopeTag_2_011918
    Immunofluorescence of His tagged plasmid transfected A549 cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) Biotin-His Mouse primary antibody (Clone 6-His). Alexa Fluor® 594 (Red) Streptavidin (Cat. No. 405240) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/30, (B, D) is 1/500 and (C) is 1/800. Concentrations for (A-C) is 1 µg/ml, (D) is 0.5 µg/ml.
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Cat # Size Price Quantity Check Availability Save
906103 100 µL 336€
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His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Host Species
Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
The antibody was purified by affinity chromatography.
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

WB - Quality tested
ICC, IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per mL (1:1000-1:2000 dilution). For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/mL (1:1000-1:2000 dilution) is recommended. For immunoprecipitation, the suggested use of this reagent is 1:150 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.

Application References
  1. Deinert K, et al. 2001. J Biol Chem. 276:6000. (WB)
  2. Kojima H, et al. 2001. J Biol Chem. 276:19238. (IF) PubMed
  3. Lapinski PE, et al. 2000. J Biol Chem. 275:6831. (WB)
  4. Bartsch D, et al. 1998. Cell. 95:211. (IF)
Product Citations
  1. Kojima H, et al. 2001. J Biol Chem. 276:19238-19243. PubMed
AB_2565057 (BioLegend Cat. No. 906103)

Antigen Details

Biology Area
Cell Biology
Gene ID
View information about 6-His on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 5    Revision Date: 09/19/2023

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Anti-6-His Epitope Tag Affinity Matrix

  • Biotin anti-6-His Epitope Tag

  • FITC anti-6-His Epitope Tag

  • Purified anti-6-His Epitope Tag

  • Direct-Blot™ HRP anti-6-His Epitope Tag

  • Alexa Fluor® 647 anti-6-His Epitope Tag


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