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Apotracker Green is a calcium-independent probe for detecting apoptotic cells. Similar to Annexin V, it detects the translocation of phosphotidylserine residues to the cell surface in a cell undergoing apoptosis. This probe can be used in conjunction with a dead cell indicator like the Helix impermeant nucleic acid stains or Zombie live/dead probes in regular cell staining buffer or PBS.Product Details
- This product consist of dry-down Apotracker™ Green and DMSO reconstitution solution.
- Apotracker™ Green is prepared by dissolving the probe in methanol and drying it down.
- Storage & Handling
- Store Apotracker™ Green at -20°C
FC - Quality tested
Live cell imaging - Validated
- Recommended Usage
Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 200-800 nM per million cells in 100 µl volume. For live cell imaging, a concentration range of 600 nM-1.0 μM is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
To reconstitute the reagent to an 80 µM stock concentration, add the following volumes:
- Add 200 µL DMSO to the 200 test size
- Add 100 µL DMSO to the 100 test size
- Add 20 µL DMSO to the 20 test size
- Take the appropriate volume of reconstitute Apotracker solution to perform a 1:10 dilution with cell staining buffer.
- Add 5 µL of the diluted reagent to 1x106 cells in 100 µL of an appropriate buffer, like FACS staining buffer, to achieve a 400nM staining solution. However, different cell types might require optimization of the staining solution concentration. We recommend a 200-800nM range for cells in suspension.
- Incubate at room temperature for 10-20 minutes.
- Co-stain with an appropriate dead cell stain as needed.
- Wash the cells at least 2 times prior to running on the cytometer. This reagent will be registered in the FITC channel of the flow cytometer.
- Gene ID