APC/Fire™ 810 anti-human CD197 (CCR7) Antibody, CD197, G043H7

Pricing & Availability

Clone: G043H7 (See other available formats)
Regulatory Status: RUO
Other Names: BLR2, CDw197, EBI1, CMKBR7
Isotype: Mouse IgG2a, κ
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Product Citations: publications

G043H7_APCFire810_CD197_CCR7_Antibody_072721 — Human peripheral blood lymphocytes were stained with anti-human CD3 Brilliant Violet 421™ and anti-human CD197 (CCR7) (clone G043H7) APC/Fire™ 810 or anti-human CD3 Brilliant Violet 421™ only.

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353263

25 tests

147€

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353264

100 tests

306€

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Description

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CCR7-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Red Laser (633 nm)
RRID: AB_2894483 (BioLegend Cat. No. 353263)
AB_2894483 (BioLegend Cat. No. 353264)

Antigen Details

Structure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution: T cells, B cells, NK, dendritic cells.
Function: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor: CCL19 and CCL21.
Cell Type: B cells, Dendritic cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610.
3. Reif K, et al. 2002. Nature 416:94.
4. Nakata B, et al. 2008. Oncology 74:69.
5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed
6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed
7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed
Gene ID: 1236 View all products for this Gene ID
UniProt: View information about CD197 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

View All CD197 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD197 (CCR7)	G043H7	FC
Alexa Fluor® 488 anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 421™ anti-human CD197 (CCR7)	G043H7	FC
PE anti-human CD197 (CCR7)	G043H7	FC
APC/Cyanine7 anti-human CD197 (CCR7)	G043H7	FC
Pacific Blue™ anti-human CD197 (CCR7)	G043H7	FC
APC anti-human CD197 (CCR7)	G043H7	FC
FITC anti-human CD197 (CCR7)	G043H7	FC
Alexa Fluor® 647 anti-human CD197 (CCR7)	G043H7	FC
PerCP/Cyanine5.5 anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 605™ anti-human CD197 (CCR7)	G043H7	FC
PE/Cyanine7 anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 711™ anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 785™ anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 510™ anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 650™ anti-human CD197 (CCR7)	G043H7	FC
PE/Dazzle™ 594 anti-human CD197 (CCR7)	G043H7	FC
Biotin anti-human CD197 (CCR7)	G043H7	FC
Purified anti-human CD197 (CCR7) (Maxpar® Ready)	G043H7	FC,CyTOF®
PerCP anti-human CD197 (CCR7)	G043H7	FC
Alexa Fluor® 700 anti-human CD197 (CCR7)	G043H7	FC
APC/Fire™ 750 anti-human CD197 (CCR7)	G043H7	FC
TotalSeq™-A0148 anti-human CD197 (CCR7)	G043H7	PG
TotalSeq™-B0148 anti-human CD197 (CCR7)	G043H7	PG
TotalSeq™-C0148 anti-human CD197 (CCR7)	G043H7	PG
Brilliant Violet 750™ anti-human CD197 (CCR7)	G043H7	FC
Ultra-LEAF™ Purified anti-human CD197 (CCR7)	G043H7	FC,Block
Spark NIR™ 685 anti-human CD197 (CCR7)	G043H7	FC
KIRAVIA Blue 520™ anti-human CD197 (CCR7)	G043H7	FC
PE/Fire™ 640 anti-human CD197 (CCR7)	G043H7	FC
Spark YG™ 581 anti-human CD197 (CCR7)	G043H7	FC
APC/Fire™ 810 anti-human CD197 (CCR7) Antibody	G043H7	FC
TotalSeq™-D0148 anti-human CD197 (CCR7)	G043H7	PG
PE/Fire™ 810 anti-human CD197 (CCR7) Antibody	G043H7	FC
PE/Cyanine5 anti-human CD197 (CCR7)	G043H7	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 05/25/2021

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