APC anti-human TCR Vα7.2 Antibody

Pricing & Availability
Clone
3C10 (See other available formats)
Other Names
T cell antigen receptor Vα7.2
Isotype
Mouse IgG1, κ
Ave. Rating
1 reviews
Product Citations
publications
3C10_APC_1_060311
Human peripheral blood lymphocytes were stained with CD3 FITC and TCR Vα7.2 (clone 3C10) APC (top), or mouse IgG1 APC isotype control (bottom).
  • 3C10_APC_1_060311
    Human peripheral blood lymphocytes were stained with CD3 FITC and TCR Vα7.2 (clone 3C10) APC (top), or mouse IgG1 APC isotype control (bottom).
  • 3C10_APC_2_060311
See APC spectral data
Cat # Size Price Quantity Avail. Save
351707 25 tests 100€
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351708 100 tests 212€
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Description

The 3C10 antibody recognizes the Vα7.2 T cell antigen receptor (TCR) α-chain segment which, joined with the Jα33 segment, constitutes an invariant TCR that is a characteristic of the mucosal-associated invariant T cells (MAIT cells). MAIT cells are restricted by a nonpolymorphic class Ib major histocompatibility complex (MHC) molecule, MHC-related molecule 1 (MR1). MAIT cells are present in human blood (1-8% of T cells), mesenteric lymph nodes, liver, and intestinal mucosa.  MAIT cells play a role in detecting and fighting off microbial infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant TCR
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Associated with an anti-CD161 or -IL18Rα staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Vα7.2  segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells.

Application References
  1. Martin E, et al. 2009. PLoS Biol. 7:525.
  2. Wakao H, et al. 2013. Cell Stem Cell 12:1. PubMed
Product Citations
  1. Cosgrove C, et al. 2013. Blood. 121:951. PubMed
  2. Wakao H, et al. 2013. Cell Stem Cell. 12:1. PubMed
  3. Cho Y, et al. 2014. J Immunol. 193:3891. PubMed
  4. Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed
  5. Sundström P, et al. 2015. J Immunol. 195: 3472 - 3481. PubMed
  6. Ling L, et al. 2016. Sci Rep. 6:20358. PubMed
  7. McWilliam H, et al. 2016. Nat Immunol. 17: 531-537. PubMed
  8. Gracey E, et al. 2016. Ann Rheum Dis. 75(12):2124-2132. PubMed
  9. Wilgenburg B, et al. 2016. Nat Commun. 7:11653. PubMed
  10. Kang S, et al. 2016. PLoS One. 10: 0004832. PubMed
  11. Dias J, et al. 2016. J Leukoc Biol. 100: 233 - 240. PubMed
  12. Keller A, et al. 2017. Nat Immunol. 18:402-411. PubMed
  13. Sattler A, et al. 2019. J Immunol. 203:2970. PubMed
  14. Leng T et al. 2019. Cell Rep. 28(12):3077-3091 . PubMed
  15. Hinks TSC, et al. 2019. Cell Rep. 28:3249. PubMed
  16. Cottineau J, et al. 2017. J Clin Invest. 127:1991. PubMed
RRID
AB_10933430 (BioLegend Cat. No. 351707)
AB_10933246 (BioLegend Cat. No. 351708)

Antigen Details

Structure
Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans
Distribution

Mucosal-associated invariant T (MAIT) cells and conventional T cells

Cell Type
T cells
Biology Area
Immunology, Adaptive Immunity
Molecular Family
TCRs
Antigen References

1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.

Gene ID
6955 View all products for this Gene ID
UniProt
View information about TCR V alpha7.2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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