Anti-RNA Polymerase TFIIB ImmunoAffinity Resin

Pricing & Availability
IIB8 (See other available formats)
Regulatory Status
Other Names
Softag 3
NeoClone Catalog# S0003
Mouse IgG2a, κ
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673701 1 mL 259€
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Softag purification takes advantage of the unique properties of a class of monoclonal antibodies, termed polyol-responsive monoclonal antibodies which can be used in immunoaffinity purification. The advantage of the Polyol-Responsive mAb System is that the target protein is not denatured during purification, so it is suitable for the purification of labile, multi-subunit protein complexes. These protein complexes bind tightly to the polyol-responsive mAbs, but they are released under very gentle elution conditions. Additionally, the proteins are highly pure compared to other systems.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
50% anti-RNA Polymerase antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
Storage & Handling
Store at 4°C until use. Use at room temperature.

IP - Quality tested

Recommended Usage

Resin binding capacity is greater than 0.3 mg/mL (based on 28 kD tagged protein). To elute, use TE, 0.7M NaCl, 30% propylene glycol.

Application Notes

Previously item# S0003 from Neoclone.

This resin is also appropriate for use in identifying proteins associated with the recombinant protein tagged with Softag 3. Softag 3 is an immunoaffinity system appropriate for purifying tagged proteins from prokaryotic expression sources. The epitope (TQDPSRVG) is derived from a subunit of eukaryotic transcription factor TFIIB.

Application References
  1. Duellman SJ, et al. 2004. Protein Expr. Purif. 35:147.

Antigen Details

TFIIB is a 35 kD human transcription factor
Biology Area
Cell Biology
Molecular Family
Nuclear Markers
Gene ID
2959 View all products for this Gene ID
View information about RNA Polymerase TFIIB on

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  • Anti-RNA Polymerase TFIIB ImmunoAffinity Resin

  • Purified anti-TFIIB


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