Anti-E. coli RNA Polymerase ImmunoAffinity Resin

Pricing & Availability
NT73 (See other available formats)
Regulatory Status
Other Names
Softag 1
NeoClone Catalog# S0001
Mouse IgG1
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673601 1 mL 259€
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E. coli RNA Core polymerase is useful in studying mechanisms of transcription initiation. E. coli Core polymerase purified with Softag 4 may be free of sigma factors found in holoenzyme.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli strain MG1655, Enterics, Borellia burgdorferi, Salmonella
Antibody Type
Host Species
50% anti- E. coli RNA Polymerase antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
Storage & Handling
Store at 4°C until use. Use at room temperature.

IP - Quality tested

Recommended Usage

Resin binding capacity is greater than 0.15 mg/mL (based on 28kD tagged protein). To elute, use TE, 0.7M NaCl, 30% propylene glycol.

Application Notes

This resin is also appropriate for use in identifying proteins associated with the recombinant protein tagged with Softag 1. Softag 1 is an immunoaffinity system appropriate for purifying tagged proteins from eukaryotic expression sources. The epitope (SLAELLNAGLGGS) is derived from a subunit of prokaryotic RNAP.

Application References

Previously item# S0001 from Neoclone.

  1. Thompson NE, et al. 2003. Anal. Biochem. 323:171.

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  • Anti-E. coli RNA Polymerase ImmunoAffinity Resin

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