Anti-BG-6 Antibody (Previously Covance catalog# SIG-3315)

Pricing & Availability
Clone
T218 (See other available formats)
Regulatory Status
RUO
Previously
Signet Catalog# 315-01
Covance Catalog# SIG-3315
Isotype
Mouse IgM
Ave. Rating
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Product Citations
publications
T218_Purified_BG-6_Antibody_IHC_032615
Staining of BG-6, T218 on formalin fixed paraffin embedded human antrum tissue.
  • T218_Purified_BG-6_Antibody_IHC_032615
    Staining of BG-6, T218 on formalin fixed paraffin embedded human antrum tissue.
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922302 1 mL 274.00€
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Description

BG-6, also known as blood group Lewis B, is found in gastrointestinal tumors, colorectal tumors, and pancreatic cancer. It is expressed in colonic epithelial cells. Blood group related antigens include A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain group of antigens. These are a group of mucin-type carbohydrate determinants found on erythrocytes, a subset of epithelial cells, and in secretions.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed against the SK-CO-10 colon cancer cell line. It is specific for the Lewis (b)(Type 1 chain).
Formulation
Ascites (in PBS with BSA + 0.1% NaN3).
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. It is recommended that the reagent be titrated for optimal performance for each application.

The optimal working dilution should be determined for each specific assay condition.
IHC: ≥1:40 for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Formalin-fixed, paraffin-embedded tissues.
Pretreatment: Not required
Incubation: 20 minutes at room temperature

Application Notes

This antibody is effective in immunohistochemistry (IHC).

Positive tissue (human): Fetal intestine

Application References
  1. Sakamoto J, et al. 1986. Cancer Res. 46:1553.
  2. Cordon-Cardo C, et al. 1986. Int J Cancer. 37:667.
  3. Cordon-Cardo C, et al. 1986. Lab Invest. 55:444.
  4. Lloyd KO. 1987. Am J Clin Path. 87:129.
Product Citations
RRID
AB_2565411 (BioLegend Cat. No. 922302)

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/18/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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