Anti-BG-5 Antibody (Previously Covance catalog# SIG-3314)

Pricing & Availability
Clone
T174 (See other available formats)
Regulatory Status
RUO
Other Names
BG5
Previously
Signet Catalog# 314-01
Covance Catalog# SIG-3314
Isotype
Mouse IgG1
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Product Citations
publications
T174_Purified_BG-5_Antibody_IHC_032615
IHC staining of anti-BG-5 antibody (clone T174) on formalin-fixed paraffin-embedded human antrum tissue. The tissue was incubated with a 1:40 dilution of the primary antibody for 20 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • T174_Purified_BG-5_Antibody_IHC_032615
    IHC staining of anti-BG-5 antibody (clone T174) on formalin-fixed paraffin-embedded human antrum tissue. The tissue was incubated with a 1:40 dilution of the primary antibody for 20 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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922202 1 mL 274.00€
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Description

BG-5, also known as blood group Lewis A, is found in gastrointestinal tumors, colorectal tumors, and pancreatic cancer. It is expressed in colonic epithelial cells. Blood group related antigens include A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain group of antigens. These are a group of mucin-type carbohydrate determinants found on erythrocytes, a subset of epithelial cells, and in secretions.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed against the SK-CO-10 colon cancer cell line. It is specific for the Lewis (a) (type 1 chain).
Formulation
Phosphate-buffered solution with BSA + 0.1% NaN3.
Preparation
The antibody was purified by ammonium sulfate precipitation.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining.  For immunohistochemistry, a dilution of 1:40 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Monteiro MA, et al. 1998. J Biol Chem. 273: 11533-43. (ELISA)
Product Citations
RRID
AB_2565410 (BioLegend Cat. No. 922202)

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Go To Top Version: 4    Revision Date: 07/25/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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