Alexa Fluor® 647 anti-RNA Polymerase II Antibody

Pricing & Availability
Clone
CTD4H8 (See other available formats)
Regulatory Status
RUO
Other Names
DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2,
Isotype
Mouse IgG1
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Product Citations
publications
CTD4H8_A647_RNAPolyII_Antibody_050318
A431 cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 647 Mouse IgG1, κ isotype ctrl antibody (negative, Cat. No. 400136) or (B-D) Alexa Fluor® 647 RNA Polymerase II antibody (clone CTD4H8) overnight at 4°C. Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (seconds) for (A-D) is 1/25. Concentrations for (A-B) is 5 µg/ml (1:100 dilution), (C) is 2 µg/ml (1:250 dilution) and (D) is 1 µg/ml (1:500 dilution).
  • CTD4H8_A647_RNAPolyII_Antibody_050318
    A431 cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 647 Mouse IgG1, κ isotype ctrl antibody (negative, Cat. No. 400136) or (B-D) Alexa Fluor® 647 RNA Polymerase II antibody (clone CTD4H8) overnight at 4°C. Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (seconds) for (A-D) is 1/25. Concentrations for (A-B) is 5 µg/ml (1:100 dilution), (C) is 2 µg/ml (1:250 dilution) and (D) is 1 µg/ml (1:500 dilution).
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
904007 25 µg 108.00€
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904008 100 µg 276.00€
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Description

RPB1 is the catalytic and largest component of RNA polymerase II, which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with RPB2, the second largest subunit. Polymerase II (Pol II) is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relatively to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft, and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single DNA template strand of the promoter is positioned within the central active site cleft of Pol II. Then, a bridging helix emanates from RPB1 and crosses the cleft near the catalytic site, which acts as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations during each neuocleotide addition. This promotes translocation of Pol II. Pol II moves on the template during transcription elongation. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II's largest subunit (RPB1), which serves as a platform for assembling factors that regulate transcription initiation, elongation, termination, and mRNA processing. It can act as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, being able to conform as both a replicate and transcriptase for the viral RNA circular genome.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Drosophilia, Yeast (S. cerevisiae), Zebrafish
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The immunogen used was a peptide containing 10 repeats of the synthetic peptide YSPTSPS using chemically synthesized phospho-ser5.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1 - 2 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

This antibody is effective in immunoblotting, immunoprecipitation and ELISA. It can also be used in chromatin immunoprecipitation assays.

The antibody CTD4H8 recognizes the C-terminal repeat of the largest subunit of RNA polymerase II from HeLa and S. cerevisiae cells. This antibody recognizes both the phosphorylated and unphosphorylated forms of the RNA polymerase II.

Based on sequence identify, this clone is liable to react with a broad range of species.

Clone CTD4H8 is also known as 4H8 or CTD 4H8.

Predicted MW is ~ 217 kD, Observed MW is on WB gel is ~240 kD

Application References
  1. Kristjuhan A, et al. 2002. Mol. Cell. 10:925.
  2. Charos AE, et al. 2012. Genome Res. 22:1668. (ChIP)
  3. Rafalska-Metcalf IU, et al. 2010. PLoS One 5:e10272. (ICC) PubMed
RRID
AB_2734674 (BioLegend Cat. No. 904007)
AB_2734675 (BioLegend Cat. No. 904008)

Antigen Details

Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
5430 View all products for this Gene ID
UniProt
View information about RNA Polymerase II on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/08/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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