Alexa Fluor® 647 anti-mouse/human A2B5 Antibody

Pricing & Availability
Clone
105 (See other available formats)
Other Names
F12A2B5, 105
Isotype
Mouse IgM, κ
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Product Citations
publications
105_A647_A2B5_Antibody_FC_103015
Insulinoma cell line, Beta-TC-6, was stained with A2B5 (clone 105) Alexa Fluor® 647 (filled histogram) or mouse IgM, κ Alexa Fluor® 647 isotype control (open histogram).
  • 105_A647_A2B5_Antibody_FC_103015
    Insulinoma cell line, Beta-TC-6, was stained with A2B5 (clone 105) Alexa Fluor® 647 (filled histogram) or mouse IgM, κ Alexa Fluor® 647 isotype control (open histogram).
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
150703 25 µg 108€
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150704 100 µg 252€
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Description

The 105/A2B5 antibody recognizes a membrane protein that is expressed by chicken retinal cells, neuronal cells, ganglia, and spinal cord. In combination with other markers, this antibody can be used to differentiate between type 1 and type 2 astrocytes. The antibody recognizes polysialogangliosides and can be used to monitor glial cell development. A2B5 antigens are expressed by a variety of tumors including glioblastomas. Tumors expressing A2B5 antigen exhibit higher recurrence. The A2B5 antibody can also be used to identify progenitor cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Embryonic chicken retinal cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.1,2

Application References
  1. Eisenbarth GS, et al. 1979. Proc. Natl. Acad. Sci. 76:4913. (IF)
  2. Eisenbarth GS, et al. 1982. Proc. Natl. Acad. Sci. 79:5066. (IF)
  3. Russell TR, et al. 1984. Cytometry 5:539. (FC)
RRID
AB_2566394 (BioLegend Cat. No. 150703)
AB_2566395 (BioLegend Cat. No. 150704)

Antigen Details

Distribution
Developing thymic epithelial cells, oligodendrocyte progenitors, neuroendocrine, embryonic neonatal tissue, astrocytoma, glioma, and squamous cell carcinoma.
Cell Type
Epithelial cells, Oligodendrocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Baracskay KL, et al. 2007. GLIA 55:1001.
2. Raff MC, et al. 1983. J. Neurosci. 6:1289.
3. Fredman P, et al. 1984. Arch. Biochem. Biophys. 2:661.
4. Xia CL, et al. 2003. Int. J. Oncol. 23:353.
5. Tchoghandjian A, et al. Brain Pathol. 20:211.

Gene ID
NA
UniProt
View information about A2B5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/18/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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