Alexa Fluor® 594 anti-rabbit IgG Antibody

Pricing & Availability
Clone
6B9G9 (See other available formats)
Other Names
N0034
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
6B9G9_A594_IgG_Antibody_011320.png
HeLa cells were fixed with cold 100% methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4 degrees without (A) or with (B-D) 1:1000 diluted purified anti-GPP130 (Poly19238) antibody followed by (A and B) 1:50 diluted (10 µg/mL), (C) 1:100 diluted (5 µg/mL) and (D) 1:200 diluted (2.5 µg/mL) Alexa Fluor® 594 anti-rabbit IgG Antibody (red) staining for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 60X objective.
  • 6B9G9_A594_IgG_Antibody_011320.png
    HeLa cells were fixed with cold 100% methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4 degrees without (A) or with (B-D) 1:1000 diluted purified anti-GPP130 (Poly19238) antibody followed by (A and B) 1:50 diluted (10 µg/mL), (C) 1:100 diluted (5 µg/mL) and (D) 1:200 diluted (2.5 µg/mL) Alexa Fluor® 594 anti-rabbit IgG Antibody (red) staining for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 60X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
410407 25 µg 100€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
410408 100 µg 236€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Technical Data Sheet (pdf)

Product Details

Reactivity
Rabbit
Antibody Type
Monoclonal
Immunogen
Rabbit IgG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
RRID
AB_2832788 (BioLegend Cat. No. 410407)
AB_2832789 (BioLegend Cat. No. 410408)

Antigen Details

Structure
IgG antibodies are large molecules of about 150 kD made of four peptide chains. It contains two identical class γ heavy chains of about 50 kD and two identical light chains of about 25 kD.
Distribution

Secreted, B cell surface

Function
Antibodies are major components of humoral immunity. IgG is the main type of antibody found in blood and extracellular fluid allowing it to control infection of body tissues. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection.
Gene ID
NA
UniProt
View information about IgG on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/13/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account