Alexa Fluor® 488 anti-human CD147 Antibody

Pricing & Availability
HIM6 (See other available formats)
Regulatory Status
VI N-L109
Other Names
Neurothelin, Basigin, EMMPRIN
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes stained with HIM6 Alexa Fluor® 488
  • a-HIM6_AF488_071607
    Human peripheral blood lymphocytes stained with HIM6 Alexa Fluor® 488
  • b-HIM6_A488_CD147_Antibody_ICC_011921
    HeLa cells were fixed with 1% paraformaldehyde (PFA) and then stained with 10 µg/ml of anti-human CD147 (clone HIM6) Alexa Fluor® 488 (green) for 3 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 20X objective.
See Alexa Fluor® 488 spectral data
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306207 25 tests 84.00€
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CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65 kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.

Product Details
Technical Data Sheet (pdf)

Product Details

Human, Cross-Reactivity: Chimpanzee, Baboon, Capuchin Monkey
Antibody Type
Host Species
Human PBMCs
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunocytochemistry, a concentration range of 5.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of T cell activation2, immunohistochemical staining1,3 of frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, and Western blotting1. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306221 and 306222).

Application References
  1. Menashi S, et al. 2003. Cancer Res. 63:7575. (WB IHC)
  2. Woodhead VE, et al. 2000. Int. Immunol. 12:1051. (Block)
  3. Reimers N, et al. 2004. Clin. Cancer Res. 10:3422. (IHC)
Product Citations
  1. Takatsu H, et al. 2011. J Biol Chem. 286:38159. PubMed
  2. Grass G, et al. 2013. J Biol Chem. 288:26089. PubMed
  3. Menck K, et al. 2015. J Mol Cell Biol. 7:143. PubMed
  4. Naito T, et al. 2015. J Biol Chem. 290:150004. PubMed
AB_528739 (BioLegend Cat. No. 306207)

Antigen Details

Ig superfamily, type I transmembrane glycoprotein, 55-65 kD

Leukocytes, erythrocytes, platelets, endothelial cells

Cell Type
Endothelial cells, Erythrocytes, Leukocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Biswas C, et al. 1995. Cancer Res. 55:434.
2. Fadool J, et al. 1993. Dev. Dyn. 196:252.
3. Felzmann T, et al. 1991. J. Clin. Immunol. 11:205.

Gene ID
682 View all products for this Gene ID
View information about CD147 on

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Go To Top Version: 5    Revision Date: 01/19/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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