Product: FITC anti-mouse Lineage Cocktail with Isotype Ctrl |
Catalog No.: 133302 |
Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital |
Staining of Mouse Bone Marrow with FITC anti-lineage Cocktail | |||
Overall: | Product Quality: | Ease of Use: | |
FITC anti-lineage cocktail works well when your cells of interest are lineage negative and PE positive. |
Experimental Design |
Application: | Flow cytometry - Surface staining |
Cells used: | Mouse bone marrow |
Brief Protocol: | Bone marrow was flushed from femurs and tibiae with PBS/2% FBS through a 27 gauge needle and a 40uM strainer. Cells were washed, counted, and stained for 20 minutes at room temperature with 20uL per test anti-lineage FITC. Cells were washed twice and analyzed on a BD LSR II. |
Results Summary: | Lineage staining was excellent. |
Additional Notes: |
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