Product: PE anti-human CD244 (2B4)

Catalog No.: 329507

Katherine Nicholas, PhD Candidate Vanderbilt University

Anti-human 2B4-PE Works Great!
Overall:	Product Quality:	Ease of Use:
2B4-PE works well to detect 2B4 expression on human T cells.

Experimental Design

Application:	Flow cytometry
Cells used:	Human PBMC
Brief Protocol:	Cyropreserved human PBMC were thawed, washed twice in PBS, and incubated overnight with or without SEB stimulation (0.1ug/mL). After 18 hours cells were washed twice in PBS and stained with a cocktail of antibodies including 2B4-PE. Cells were incubated with antibodies for 15 minutes at room temperature and 0.25uL 2B4-PE was used to stain 1 million cells in 200uL PBS. 2B4-PE expression was measured on live T cells and expression was detected with and without stimulation.
Results Summary:	2B4-PE worked well to detect 2B4+ T cells.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

See all Reviews