Application: |
Detection of IL-1β release from microglia |
Cells used: |
Microglia |
Brief Protocol: |
We generated mixed glial cell cultures from neonatal mice (p0-2) in 24-well plates and stimulated the cells for 2h with LPS to induce production of pro-IL-1β in microglia. We subsequently stimulated cells with 1.5mM ATP to induce P2X7-mediated inflammasome formation and cleavage of pro-IL-1β and release of IL-1β into the supernatant. Latter was quantified using Mouse IL-1β ELISA MAX™ Deluxe. |
Results Summary: |
Supernatants contained 100-300pg/ml mouse IL-1β, depending on the number of microglia in the mixed glial cell culture. |
Additional Notes: |
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