Product: APC/Cyanine7 anti-mouse/human CD45R/B220 |
Catalog No.: 103224 |
Adam Coleman, Postdoctoral Fellow Oak Ridge Institute for Science and Education |
Strong, Specific Signal (APC/Cyanine7 anti-mouse/human CD45R/B220) | |||
Overall: | Product Quality: | Ease of Use: | |
The antibody clearly stains mouse splenic B cells. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Mouse splenocytes |
Brief Protocol: | Mash mouse spleens through nylon mesh Wash cells in FACS buffer (1x PBS + 2%FBS + 2mM EDTA) Resuspend cells in 100 ul FACS buffer + 1 ul APC/Cyanine7 anti-mouse CD45R/B220 on ice for 10 minutes Wash cells in FACS buffer and analyze on flow cytometer |
Results Summary: | Splenic B cells are strongly positive for B220 |
Additional Notes: |
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