Product: Brilliant Violet 785™ anti-human CD25 |
Catalog No.: 302638 |
Joy Hsu, Specialist UCSF |
Anti-human CD25 Brilliant Violet 785™ | |||
Overall: | Product Quality: | Ease of Use: | |
Stained for IL-2Ra on T cell compartment. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Human tumor infiltrate |
Brief Protocol: | Cell suspension was plated in 96-well plate at 2-4 million cells/ well. Antibody was added in proper flow buffer at 1:100 for 30 minutes in the dark at 4 degrees Celsius. Used in conjunction with anti-human CD4 to parse out CD4+ T cells and "activated" T cells from naive and CD8+ cells. |
Results Summary: | Functional staining for IL-2Ra. |
Additional Notes: |
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