Product: APC anti-mouse CD5

Catalog No.: 100625

Andreia Lino, PhD Deutsches Rheuma-Forschungszentrum, a Leibniz Institute

anti-mouse CD5 APC for Flow Cytometry
Overall:	Product Quality:	Ease of Use:
Very good and bright antibody.

Experimental Design

Application:	Flow cytometry
Cells used:	Mouse peritoneal cavity
Brief Protocol:	Resuspend in PBS/BSA and count the cells. Load up to 5x106 cells onto a V-Bottom 96-well-plate. Spin down, resuspend in 50uL of PBS/BSA containing αFcγR (2.4G2) 30 µg/ml and incubate 15 minutes at 4°C. Spin down and resuspend in 50 µl of antibody staining solution containing CD5APC (1:300). Incubate 15min at 4°C in the dark. Wash with PBS/BSA, spin down resuspend and transfer to FACS tubes to read. Use PI to exclude the dead cells.
Results Summary:	T cells are higher expressors of CD5, while B cells (subset of B-1a cells) that are enriched in the peritoneal cavity are intermediate expressors of CD5.
Additional Notes:	This antibody allows discriminate T cells and B cells very well.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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