Application: |
Flow Cytometry |
Cells used: |
Mouse Splenocytes |
Brief Protocol: |
Isolate splenocytes. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice. Wash cells once in FACS buffer. Stain with isotype control or mouse CD1d PE antibody (1:500 dilution, 100 uL per million cells) for 20 minutes on ice. Wash cells once in FACS buffer. Resuspend cells in 100-250 uL FACS buffer containing 1.5% paraformaldehyde. |
Results Summary: |
Mouse splenic B cell CD1d expression was easily visualized. |
Additional Notes: |
Mouse splenic B cell CD1d expression directly ex vivo (left panel) or following 5 hour LPS stimulation (right panel) was used to phenotype spleen B cell populations relative to CD5 expression. |
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