Product: PE anti-mouse CD71 |
Catalog No.: 113807 |
Andreia Lino, PhD Deutsches Rheuma-Forschungszentrum, a Leibniz Institute |
Anti-mouse CD71 PE for Flow Cytometry | |||
Overall: | Product Quality: | Ease of Use: | |
Very good option to stain CD71. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Mouse splenocytes |
Brief Protocol: | Resuspend in PBS/BSA and count the cells. Load up to 5x106 cells onto a V-Bottom 96-well-plate. Spin down, resuspend in 50uL of PBS/BSA containing αFcγR (2.4G2) 30 µg/ml and incubate 15min at 4°C. Spin down and resuspend in 50 µl antibody staining solution containing anti-CD71 PE (1:200). Incubate 15min at 4°C in the dark. Wash twice with PBS/BSA, resuspend and transfer to FACS tubes to read. Use PI to exclude the dead cells. |
Results Summary: | CD71 is expressed B cells in the spleen. |
Additional Notes: | This antibody is superior to the biotin one, although they are the same clone. |
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