Product: PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1) |
Catalog No.: 108113 |
Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital |
Staining Mouse Bone Marrow with anti-mouse Sca-1 PE/Cyanine7 | |||
Overall: | Product Quality: | Ease of Use: | |
Anti-mouse Sca-1 PE/Cyanine7 works well on mouse bone marrow. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Mouse bone marrow |
Brief Protocol: | Bone marrow was flushed from femurs and tibiae with PBS/2%FBS through a 27 gauge needle and a 40uM strainer. Cells were washed, counted, and stained for 15 minutes at room temperature with the recommended dilution of anti-Sca1 PE/Cyanine7. Cells were washed twice and analyzed on a BD LSR II. |
Results Summary: | Sca-1 staining was as expected, compared to isotype control. |
Additional Notes: |
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