Validation

Veri-Cells™ PBMC have been validated with LEGENDScreen™ Human Cell Screening (PE) Kit and have been shown to stain positive for approximately over 150 surface markers. Transcription factors like FOXP3 and Helios were also tested and stained well with Veri-Cells™ PBMC using our True-Nuclear™ Transcription Factor Buffer Set.

Excellent Surface Staining

Excellent Surface Staining

Transcription Factor Staining

Transcription Factor Staining

150+ Surface Markers Validated on Veri-Cells™ PBMC

The following cell surface markers were validated using BioLegend's LEGENDScreen™ Human PE kit.

Marker	Clone
CD1c	L161
CD1d	51.1
CD2	RPA-2.10
CD3	HIT3a
CD4	RPA-T4
CD5	UCHT2
CD6	BL-CD6
CD7	CD7-6B7
CD8a	HIT8a
CD9	HI9a
CD11a	HI111
CD11b	ICRF44
CD11b	CBRM1/5
CD11c	3.9
CD13	WM15
CD14	M5E2
CD16	3G8
CD18	TS1/18
CD19	HIB19
CD20	2H7
CD21	Bu32
CD22	HIB22
CD23	EBVCS-5
CD24	ML5
CD26	BA5b
CD27	O323
CD28	CD28.2
CD29	TS2/16
CD31	WM59
CD32	FUN-2
CD33	WM53
CD35	E11
CD36	5-271
CD38	HIT2
CD39	A1
CD40	HB14
CD41	HIP8
CD42b	HIP1
CD43	CD43-10G7
CD44	BJ18

Marker	Clone
CD45	HI30
CD45RA	HI100
CD45RB	MEM-55
CD45RO	UCHL1
CD46	TRA-2-10
CD47	CC2C6
CD48	BJ40
CD49d	9F10
CD49e	NKI-SAM-1
CD49f	GoH3
CD50	CBR-IC3/1
CD52	HI186
CD53	HI29
CD54	HA58
CD55	JS11
CD56	HCD56
CD57	HCD57
CD58	TS2/9
CD59	p282 (H19)
CD61	VI-PL2
CD63	H5C6
CD64	10.1
CD69	FN50
CD73	AD2
CD74	LN2
CD79b	CB3-1
CD81	5A6
CD82	ASL-24
CD84	CD84.1.21
CD85	MKT5.1
CD85d	42D1
CD85	GHI/75
CD86	IT2.2
CD87	VIM5
CD88	S5/1
CD89	A59
CD93	VIMD2
CD94	DX22
CD95	DX2
CD97	VIM3b

Marker	Clone
CD99	HCD99
CD100	A8
CD101	BB27
CD102	CBR-IC2/2
CD116	4H1
CD122	TU27
CD123	6H6
CD124	G077F6
CD126	UV4
CD127	A019D5
CD132	TUGh4
CD134	Ber-ACT35 (ACT35)
CD148	A3
CD154	24-31
CD156c	SHM14
CD158a/h	HP-MA4
CD158b	DX27
CD158e1	DX9
CD161	HP-3G10
CD162	KPL-1
CD163	GHI/61
CD164	67D2
CD165	SN2 (N6-D11)
CD172a	SE5A5
CD172b	B4B6
CD172g	LSB2.20
CD180	MHR73-11
CD182	5E8/CXCR2
CD196	G034E3
CD197	G043H7
CD200	OX-104
CD200R	OX-108
CD226	11A8
CD229	Hly-9.1.25
CD244	C1.7
CD268	11C1
CD272	MIH26
CD277	BT3.1
CD278	C398.4A

Marker	Clone
CD279	EH12.2H7
CD284	HTA125
CD290	3C10C5
CD298	LNH-94
CD300e	UP-H2
CD300F	UP-D2
CD314	1D11
CD319	162.1
CD328	6-434
CD335	9E2
CD337	P30-15
CD352	NT-7
CD354	TREM-26
β2-microglobulin	2M2
C3aR	hC3aRZ8
CLEC12A	50C1
CX3CR1	2A9-1
FcRL6	2H3
HLA-A,B,C	W6/32
HLA-A2	BB7.2
HLA-DQ	HLADQ1
HLA-DR	L243
HLA-E	3D12
HVEM	122
IgD	IA6-2
IgM	MHM-88
Integrin β7	FIB504
NKp80	5D12
Siglec-9	K8
TCR gamma/delta	B1
TCR Vβ13.2	H132
TCR Vβ23	αHUT7
TCR α/β	IP26
TCR Vβ8	JR2 (JR.2)
TCR Vβ9	MKB1
TCR Vδ2	B6
TCR Vγ9	B3
TCR Vα7.2	3C10

100+ Surface Markers Validated on Veri-Cells™ Leukocytes

The following cell surface markers were validated using LEGENDScreen™ Human PE kit.

Marker	Clone
β2-microglobulin	2M2
CD1c	L161
CD1d	51.1
CD2	RPA-2.10
CD3	HIT3a
CD4	RPA-T4
CD5	UCHT2
CD6	BL-CD6
CD10	HI10a
CD11a	HI111
CD11b	ICRF44
CD13	WM15
CD14	M5E2
CD15	W6D3
CD15 (SSEA-1)	MC-480
CD16	3G8
CD18	TS1/18
CD19	HIB19
CD20	2H7
CD21	Bu32
CD22	HIB22
CD23	EBVCS-5
CD24	ML5
CD26	BA5b
CD27	O323
CD28	CD28.2
CD29	TS2/16
CD31	WM59
CD32	Fun-2
CD33	WM53

Marker	Clone
CD35	E11
CD38	HIT2
CD39	A1
CD40	HB14
CD43	CD43-10G7
CD44	BJ18
CD45	HI30
CD45RA	HI100
CD45RB	MEM-55
CD45RO	UCHL1
CD47	CC2C6
CD48	BJ40
CD49d	9F10
CD49e	NKI-SAM-1
CD50	CBR-IC3/1
CD52	HI186
CD53	HI29
CD55	JS11
CD56	HCD56
CD57	HCD57
CD58	TS2/9
CD59	p282 (H19)
CD64	10.1
CD66a/c/e	ASL-32
CD66b	G10F5
CD7	CD7-6B7
CD74	LN2
CD81	5A6
CD82	ASL-24
CD84	CD84.1.21

Marker	Clone
CD85j	GHI/75
CD86	IT2.2
CD87	VIM5
CD88	S5/1
CD89	A59
CD8a	HIT8a
CD94	DX22
CD95	DX2
CD97	VIM3b
CD99	HCD99
CD101	BB27
CD102	CBR-IC2/2
CD116	4H1
CD122	TU27
CD127	A019D5
CD132	TUGh4
CD141	M80
CD155	SKII.4
CD156c	SHM14
CD161	HP-3G10
CD163	GHI/61
CD164	67D2
CD172a/b	SE5A5
CD172b	B4B6
CD172g	LSB2.20
CD180	MHR73-11
CD197	G043H7
CD200	OX-104
CD226	11A8
CD244	C1.7

Marker	Clone
CD245	DY12
CD263	DJR3
CD268	11C1
CD278	C398.4A
CD279 (PD-1)	EH12.2H7
CD284	HTA125
CD298	LNH-94
CD300e	UP-H2
CD300F	UP-D2
CD314	1D11
CD319	162.1
CD328	6-434
CD335	9E2
CD337	P30-15
CD354	TREM-26
CLEC12A	50C1
CX3CR1	2A9-1
DcR1 (TRAIL-R3)	DJR3
Galectin-3 (Mac-2)	Gal397
HLA-A,B,C	W6/32
HLA-DQ	HLADQ1
HLA-DR	L243
IgD	IA6-2
LTβR	31G4D8
NKp80	5D12
Siglec-9	K8
TCR α/β	IP26
TCR gamma/delta	B1
TCR Vδ2	B6
Vγ9	B3