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Protocols

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Welcome to BioLegend's interactive Technical Protocols. These protocols provide step-by-step instructions on running your desired lab application. For those interested in running the protocol in real time, we provide a timer that is pre-set with the appropriate count down time for that step. We also provide checkboxes to help keep track of completed steps. Relevant BioLegend products are linked, so just click on the relevant catalog number to get to the data sheets. General tips and FAQs that will provide pointers along the way are also included. Contact Technical Service to get help with any of these protocols.

 

AcuityADVANCED Protocol
Ce3D™ Tissue Clearing Kit Protocol
Immunohistochemistry Protocol for Frozen Sections
Immunohistochemistry Protocol for Beta Amyloid Products using USA Detection Kit
Immunohistochemistry Protocol for Keratin Antibodies
Immunohistochemistry Protocol for Ultra Streptavidin Detection Kits USA
Immunocytochemistry Staining Protocol
Immunocytochemistry Staining for Methanol Fixed Cells
Immunohistochemistry Protocol for Paraffin-Embedded Sections
Immunofluorescent Staining of Foxp3 in Frozen Sections

For previous versions of existing protocols or to access protocols that have been removed, please contact technical service.

 

Bulk Epitope and Nucleic Acid Sequencing (BEN-seq) Protocol
TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3.1 (Dual Index) Protocol
TotalSeq™-B or -C with 10x Feature Barcoding Technology
TotalSeq™-D with Mission Bio Tapestri® Single-Cell DNA + Protein Protocol
TotalSeq™ Antibody Cocktail “Spike-in” Guidance
TotalSeq™-B Cell Surface and Intracellular Cytoplasmic Protein Staining with 10x Chromium Single Cell Gene Expression Flex
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