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Our antibodies are compatible with a variety of multiplexed spatial protein analysis platforms and techniques. These include both commercially available and community-developed (open) applications.
The following table provides a current snapshot of our reagents that have been reported to work with each platform or technique. The reported compatibility of our clones highlighted below originates from the commercial vendor or community listed for each method. Please note, since we have not verified these methods in-house, we will only be able to provide limited support on these applications.
|
Method |
Instrument |
Company |
Detection Method (Read-out) |
Number of Targets Per Assay (Plexity) |
Recommended and Screened Targets |
Supported Sample Types |
|
Cyclic Imaging |
Phenocycler/ PhenoImager |
Akoya |
Fluorophores |
40 typical, 100 possible |
206 Akoya-screened purified antibodies
Conjugation kits are available from Akoya
|
FFPE, FF |
|
T-CycIF |
Standard fluor-imagers |
Open-source (standard imaging systems) |
Fluorophores |
Up to 60 |
21 recommended antibodies (including conjugated and purified clones)
|
FFPE |
|
ChipCytometry |
CellScape/ Zellscanner |
Canopy/ Bruker |
Fluorophores |
21 |
Canopy-recommended target list
Customers will need to validate specific clones |
FFPE, FF |
|
SeqIF |
COMET™/LabSat® |
Lunaphore |
Fluorophores (uses secondary fluor-conjugated antibodies) |
40-60 (COMET™) 10 (LabSat®) |
Qualified and verified targets from Lunaphore
Customers will need to validate specific primary antibody clones |
FFPE, FF |
|
Imaging Mass Cytometry |
Hyperion™ (Helios™+ Imager) |
Standard BioTools (formerly Fluidigm) |
Metals (mass spectrometry) |
40-plex commercially available (100-plex possible) |
Conjugation kit available from Standard BioTools
Publication using 7 BioLegend antibodies
Customers will need to validate clones
|
FFPE, FF |
|
MIBI™ |
MIBIScope™ |
Ionpath |
Metals (mass spectrometry) |
40 |
A conjugation kit is available from Ionpath
Customers will need to validate clones
|
FFPE, FF |
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