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PE anti-BATF Antibody

Pricing & Availability
Clone
9B5A13 (See other available formats)
Regulatory Status
RUO
Other Names
B-cell-activating transcription factor, Basic leucine zipper transcriptional factor ATF-like, SFA-2, BATF1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
9B5A13_PE_BATF_Antibody_FC_051414.jpg
CD3+ CD28- stimulated (3 days) human peripheral blood lymphocytes were treated with FOXP3 Fix/Perm Buffer Set and then intracellularly stained with BATF (clone 9B5A13) PE (filled histogram). Open histogram represents staining with non-stimulated cells.
  • 9B5A13_PE_BATF_Antibody_FC_051414.jpg
    CD3+ CD28- stimulated (3 days) human peripheral blood lymphocytes were treated with FOXP3 Fix/Perm Buffer Set and then intracellularly stained with BATF (clone 9B5A13) PE (filled histogram). Open histogram represents staining with non-stimulated cells.
See PE spectral data
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654803 25 tests £105
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654804 100 tests £263
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Description

BATF is a basic leucine zipper transcription factor belonging to the ATF superfamily. BATF forms heterodimers with Jun proteins, which in turn binds to AP-1 consensus sites. The BATF/Jun heterodimers have significantly reduced transcriptional activity compared to Fos/Jun heterodimers. Therefore, induction of BATF expression suppresses AP-1 target genes by competing for Fos/Jun binding sequences. BATF is expressed at low levels in hematopoietic cells and can be induced in response to a variety of stimuli. BATF knockout mice show defects in cytokine expression and in the differentiation of T helper cell subsets and B cells, but not in the proliferation of lymphocytes. These findings suggest that BATF is a tissue-specific regulator for AP-1 activity and plays an essential role in the regulation of immune responses. During early EBV infection, BATF expression is induced by EBNA2 proteins, subsequently preventing infected B cells from entering apoptotic and lytic pathways. This indicates that BATF functions as a modulator to maintain latency status of virus-infected cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human BATF recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
RRID
AB_2563516 (BioLegend Cat. No. 654803)
AB_2563517 (BioLegend Cat. No. 654804)

Antigen Details

Structure
14 kD protein containing a bZIP (basic-leucine zipper) domain, which mediates dimerization with Jun family members.
Distribution

Nucleus. Expressed most abundantly in hematopoietic tissues.

Function
AP-1 transcription complexes regulate gene expression in response to various stimuli, such as cytokines, growth factors, stress, and infection. BATF belongs to the AP-1/ATF superfamily and negatively regulates AP-1 mediated transcription when it forms heterodimers with Jun proteins.
Interaction
Forms heterodimers with c-Jun, JunB, and JunD. Interacts with IFI35, IRF4, and IRF8 through bZIP domain.
Cell Type
B cells, Dendritic cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Betz BC, et al. 2010. J. Exp. Med. 207:933.
2. Dorsey MJ, et al. 1995. Oncogene 11:2255.
3. Echlin DR, et al. 2000. Oncogene 19:1752.
4. Johansen LM, et al. 2003. J. Virol. 77:6029.
5. Schraml BU, et al. 2009. Nature 460:405.
6. Tussiwand R, et al. 2012. Nature 490:502.

Gene ID
10538 View all products for this Gene ID
UniProt
View information about BATF on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05/28/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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