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Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development. Abnormal hyperphosphorylation, aggregation, and toxic gain of function of tau are associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Phosphorylated tau at serine 262 is commonly associated with AD legion sites. Recent studies indicate that cerebrospinal fluid tau phosphorylated at threonine 181 has diagnostic utility for several neurological disorders including AD. The Tau Antibody Sampler Kit provides flexibility for sampling and detection of phosho-specific species of tau and total tau in human tissue.Product Details
- Kit Contents
Specificity Format Clone Size Reactivity Isotype Anti-Tau Phospho (Ser262) Purified A15091A 25 μg Human Mouse IgG2b, κ Anti-Tau Phospho (Thr181) Purified M7004D06 25 μg Human Mouse IgG1, κ Anti-Tau, 1-223 Purified A16103A 25 μg Human Rat IgG2b, κ Anti-Tau, 368-441 Purified A16097F 25 μg Human Rat IgG2a, κ
* For detailed information about each specificity, please refer to the datasheets of the individual products.
- Please refer to individual product datasheets of the purified formats for details.
- All antibodies in this kit were purified by affinity chromatography.
- Storage & Handling
- Upon receipt, store undiluted at 2-8°C.
IHC-P, WB - Quality tested
- Recommended Usage
Each lot of antibodies in this kit is quality control tested by immunohistochemical staining on formalin-fixed paraffin-embedded tissue or Western blotting. For immunohistochemistry, the suggested uses of these reagents are as follows:
Anti-Tau Phospho (Ser262): 5.0 - 10.0 µg/ml
Anti-Tau Phospho (Thr181): 1.0 - 10.0 µg/ml
For Western blotting, the suggested uses of these reagents are as follows:
Anti-Tau, 1-223: 1.0 - 5.0 µg/ml
Anti-Tau, 368-441: 1.0 - 5.0 µg/ml
It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
For validated or reported applications for these antibodies, please see individual product datasheets.
AB_2734652 (BioLegend Cat. No. 899902)
- Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: Cytoskeleton, nucleus, plasma membrane, and cytosol.
- Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
- Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Phospho-Proteins, Tau
- Antigen References
1. Frontini M, et al. 2009. Nucleic Acids Res. 37:1073.
2. Yarilina A, et al. 2008. Nat. Immunol. 9:378.
3. Hayashi H, et al. 2011. Proc. Natl. Acad. Sci. USA. 108:18766.
4. Hida S, et al. 2005. Blood. 106:2011.
5. Lin R, et al. 1999. Mol. Cell Biol. 19:959.
6. Hiscott J, et al. 2007. J. Biol. Chem. 282:15325.
7. Lu R. 2008. Trends Immunol 29:487.
8. Barnes BJ, et al. 2003. J. Biol. Chem. 278:16630.
9. Rullo OJ, et al. 2010. Ann. Rheum. Dis. 69:611.
10. Botti E, et al. 2011. Proc. Natl. Acad. Sci. USA 108:13710.
11. Restivo G, et al. 2011. EMBO 30:4571.
12. Yu Y, et al. 2010. Immunity. 33:863.
13. Liang Q, et al. 2011. J. Immunol. 186:1001.
14. Ouyang X, et al. 2011. Nat. Commun. 2:314.
15. Thibault DL, et al. 2008. J. Clin. Invest. 118:1417.
16. Kraus TA, et al. 2003. J. Biol. Chem. 278:13033.
17. Xiao W, et al. 2001. J. Biol. Chem. 276:23275.
- Gene ID
- 4137 View all products for this Gene ID
- View information about Tau on UniProt.org