APC anti-human CD137 (4-1BB) Antibody

Pricing & Availability
Clone
4B4-1 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-7
Other Names
4-1BB, ILA, CD137, TNFRSF9
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4B4-1_APC_CD137_Antibody_051018
PHA-stimulated (3 days) human peripheral blood mononuclear cells stained with CD137 (clone 4B4-1) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • 4B4-1_APC_CD137_Antibody_051018
    PHA-stimulated (3 days) human peripheral blood mononuclear cells stained with CD137 (clone 4B4-1) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
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309809 25 tests £99
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309810 100 tests £228
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Description

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ectodomain of recombinant human 4-1BB fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
  2. Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
  3. Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
  4. Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
Product Citations
  1. Apostolidis SA, et al. 2021. Nat Med. 27:1990. PubMed
  2. Nielsen MA, et al. 2022. Front Immunol. 13:915890. PubMed
  3. Tarke A, et al. 2021. Cell Reports Medicine. 2(2):100204. PubMed
  4. Luo X, et al. 2020. Front Immunol. 11:623. PubMed
  5. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
  6. Stevenson EM, et al. 2022. Nat Commun. 13:4888. PubMed
  7. van der Ploeg K, et al. 2022. Cell Rep Med. 3:100640. PubMed
  8. Grifoni A, et al. 2020. J Virol. 94:00:00. PubMed
  9. Qiu C, et al. 2022. Front Immunol. 12:764949. PubMed
  10. Yarmarkovich M, et al. 2021. Nature. 599:477. PubMed
  11. You M, et al. 2021. Nat Cell Biol. 23:620. PubMed
  12. Painter MM, et al. 2021. Immunity. 54:2133. PubMed
  13. Zhang J, et al. 2022. Nature. 609:369. PubMed
  14. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  15. Tarke A, et al. 2021. Cell Reports Medicine. 2(7):100355. PubMed
  16. Tarke A, et al. 2022. Cell. 185:847. PubMed
  17. Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed
  18. Grifoni A, et al. 2020. Cell. 181(7):1489-1501.e15.. PubMed
  19. Valenzuela NM, et al. 2021. Front Immunol. 12:648946. PubMed
  20. Peluso MJ, et al. 2021. Cell Rep. 36:109518. PubMed
  21. Voic H, et al. 2020. J Virol. 94: . PubMed
  22. Kishton RJ, et al. 2022. Cell Rep. 40:111153. PubMed
  23. Yu ED, et al. 2022. Cell Host Microbe. 30:1269. PubMed
  24. Tarke A, et al. 2022. Int J Mol Sci. 23:. PubMed
  25. Mo F, et al. 2021. Nat Biotechnol. 56:39. PubMed
  26. Martin SD, et al. 2018. Oncoimmunology. 7:e1371895. PubMed
  27. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  28. Lederer K, et al. 2022. Cell. . PubMed
  29. Schouest B, et al. 2021. J Virol. 95:. PubMed
  30. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  31. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  32. Eriksen LL, et al. 2021. PLoS One. 16:e0255574. PubMed
  33. Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed
  34. Yu ED, et al. 2022. Cell Host Microbe. . PubMed
  35. Nuffel A, et al. 2012. J Immunol Methods. 377:23. PubMed
  36. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID
AB_830671 (BioLegend Cat. No. 309809)
AB_830672 (BioLegend Cat. No. 309810)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 30 kD
Distribution

Activated T cells

Function
T cell costimulation
Ligand/Receptor
4-1BB ligand
Cell Type
T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Gruss H, et al. 1995. Blood 85:3378.
2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355.
3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219.
4. Schwarz H, et al. 1996. Blood 87:2839.

Gene ID
3604 View all products for this Gene ID
UniProt
View information about CD137 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/10/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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