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Veri-Cells™ Activated (Surface) PBMC

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Regulatory Status
RUO
Other Names
Control Cells
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Veri-cells_Activated_surface_PBMC_1_051518_updated
Veri-Cells™ Activated (surface) PBMC stained with CD3 APC/Fire™ 750, CD4 FITC, and CD279 (PD-1) PE/Cy7 or CD366 (TIM3) PE (left). Plots were generated on CD3+ cells and gated based on corresponding isotype control stains (right).
  • Veri-cells_Activated_surface_PBMC_1_051518_updated
    Veri-Cells™ Activated (surface) PBMC stained with CD3 APC/Fire™ 750, CD4 FITC, and CD279 (PD-1) PE/Cy7 or CD366 (TIM3) PE (left). Plots were generated on CD3+ cells and gated based on corresponding isotype control stains (right).
  • Veri-cells_Activated_surface_PBMC_2_051518_updated
    Veri-Cells™ Activated (surface) PBMC stained with CD3 APC/Fire™ 750, CD4 FITC, and CD25 PerCP/Cy5.5 or CD69 Alexa Fluor® 700 (left). Plots were generated on CD3+ cells and gated based on corresponding isotype control stains (right).
  • Veri-cells_Activated_surface_PBMC_3_051518_updated
    Veri-Cells™ Activated (surface) PBMC stained with CD3 APC/Fire™ 750, CD4 FITC, and CD134 (OX40) APC or TIGIT (VSTM3) PE/Dazzle™ 594 (left). Plots were generated on CD3+ cells and gated based on corresponding isotype control stains (right).
  • Veri-cells_Activated_surface_PBMC_4_051518_updated
    Veri-Cells™ Activated (surface) PBMC scatter profile (left) and stain for CD3 APC/Fire™ 750 (right).
Cat # Size Price Quantity Check Availability Save
426701 10 tests £72
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426703 50 tests £298
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Description

The reconstituted lyophilized human Activated (surface) PBMC can be used for immunofluorescent staining and multi-color flow cytometry assays. The cells can be used as activated cell marker controls with minimal variability across experiments. These cells have been verified to work with commonly tested cell surface, as well as T cell activation markers such as CD3, CD4, CD8, CD25, CD366(TIM3), TIGIT, CD69, CD279 and CD134.

This product contains human cells, a potentially biohazardous material. Blood used in preparation of these samples was tested and found to be negative for antibody and nucleic acid testing against Human Immunodeficiency virus (HIV), Hepatitis B and C virus and HTLV (Human T-Lymphotrophic virus I and II), surface antigen for Hepatitis B (HBsAg) virus and syphilis and West Nile Virus using FDA approved methods. Biological tests are not 100% accurate. Use standard precautions when handling, and treat as if the product is capable of transmitting disease. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen guidelines.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

Cat. No. 426701

  • 2 vials of Veri-Cell™ Activated (surface) PBMC (≥1 X 106 cells per vial) 
  • 1 vial of 0.7 ml Veri-Cells™ Buffer A Plus

Cat. No. 426703

  • 10 vials of Veri-Cell™ Activated (surface) PBMC (≥1 X 106 cells/vial)
  • 5 vials of 0.7 ml Veri-Cells™ Buffer A Plus
     

Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.

Product Details

Formulation
Cat. No. 426701: 2 vials each containing ≥1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).
Cat. No. 426703: 10 vials each containing ≥1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).
Preparation
The Veri-Cells™ PBMC Kit is prepared from 2 lyophilized human Activated (surface) PBMC and a vial of Veri-Cells™ Buffer A Plus. Veri-Cells Activated (surface) PBMC contain lyophilized PBMCs that had been stimulated using Concanavalin A (ConA) for 72 hours.
Storage & Handling
Store the kit at 2°C - 8°C upon receipt. Do not open sealed pouch until ready to use. Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided. Contact BioLegend Technical Support for information on the current lots
Application

FC - Quality tested
Recommended Usage

50 µl per test (after reconstitution with 325 µl of Veri-Cells™ Buffer A Plus)

Note: Veri-Cells™ Buffer A Plus may show precipitation over time, however this is normal and does not affect performance of the buffer.

Application Notes

The Veri-Cells™ Activated (surface) PBMC Kit can be used as a positive control for detecting T cell activation markers such as CD25, CD69, CD134, CD279, CD366 (TIM3) and TIGIT (VSTM3). Assessing the suitability of using this product as a control for end user’s molecule of interest is recommended, as each lot is verified based on the expression levels of markers mentioned above. For best results, Cell Staining Buffer is recommended for any washing steps. Product performance specifications are based on testing with BioLegend reagents and protocols. Deviations from the use of these reagents and procedures have not been assessed and may affect performance.

Antibodies Used for Product Validation

Target

Clone

Fluorophore

CD3

SK7

APC/Fire™ 750

CD4

RPA-T4

FITC

CD25

M-A251

PerCP/Cyanine5.5

CD366/TIM3

F38-2E2

PE

TIGIT

A15153G

PE/Dazzle™ 594

CD69

FN50

Alexa Fluor® 700

CD279

EH12.2H7

PE/Cy7

CD134

Ber-ACT35

APC
 
 

Cell subset

Acceptance Criteria, % positive of lymphocyte gate

CD3+CD25+

>10%

CD3+CD69+

>5%

CD3+ CD134 (OX40)+

>5%

CD3+ CD366 (Tim-3)+

>1%

CD3+TIGIT (VSTM3)+

>10%

CD3+CD279 (PD-1)+

>8%

Note on indicated ranges: Percent positive values are based on gates set on cell stained with corresponding isotype for each marker. The antibodies used for testing were the following: CD3 APC/Cy7 (clone SK7), CD25 PerCP/Cy5.5 (clone M-A251), CD134 (OX40) APC (clone Ber-ACT35), CD69 Alexa Fluorâ 700 (clone FN50), CD279 (PD-1) PE/Cy7 (clone EH12.2H7), CD366 (Tim-3) PE clone (F38-2E2), TIGIT (VSTM3) PE/Dazzle 594™ (clone A15153G). Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. The Certificate of Analysis specific values are provided as a reference. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.

Please use our CoA Look-Up Tool to find the expected lot-specific frequencies of certain cell populations included within each lot of Veri-Cells™ Activated (Surface) PBMC. 

Additional Product Notes

Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 06/03/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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