Ultra-LEAF™ Purified anti-mouse CD27 Antibody

Pricing & Availability
Clone
A18209D (See other available formats)
Other Names
CD27 antigen, CD antigen 27, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7, TNFRSF7T-cell activation antigen CD27
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A18209D_ULEAF_CD27_Antibody_031621
Recombinant mouse CD27L (Cat. No. 550406) (black circles) binds to immobilized recombinant mouse CD27 (TNFRSF7)-Fc chimera (Cat. No. 765604). Ultra-LEAF™ purified anti-mouse CD27 antibody (clone A18209D) (purple squares) inhibits the binding in a dose-dependent manner whereas the GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.08 µg/mL recombinant mouse CD27L to 2 µg/mL immobilized recombinant mouse CD27 (TNFRSF7)-Fc chimera. ND50 = 0.3 - 1.5 µg/mL.
  • A18209D_ULEAF_CD27_Antibody_031621
    Recombinant mouse CD27L (Cat. No. 550406) (black circles) binds to immobilized recombinant mouse CD27 (TNFRSF7)-Fc chimera (Cat. No. 765604). Ultra-LEAF™ purified anti-mouse CD27 antibody (clone A18209D) (purple squares) inhibits the binding in a dose-dependent manner whereas the GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.08 µg/mL recombinant mouse CD27L to 2 µg/mL immobilized recombinant mouse CD27 (TNFRSF7)-Fc chimera. ND50 = 0.3 - 1.5 µg/mL.
Cat # Size Price Quantity Avail. Save
942003 100 µg £133
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942004 1 mg £513
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Description

CD27 (TNFRSF7) is a member of the TNFR family. It is a type I membrane glycoprotein with a MW of 55 kD that forms a disulfide‑linked homodimer. The receptor possesses three cysteine rich repeats in the extracellular domain, the transmembrane region, and the intracellular domain. CD27 binds CD27L (CD70), another member of the TNF ligand family that forms a trimer; thus, it has been speculated that the CD27L binds to a trimeric structure composed by three dimeric CD27 molecules. The interaction between CD27 and its ligand, CD70, plays a role in providing co-stimulation for prolonged lymphocyte survival, enhanced T-cell proliferation, and memory-cell formation. T cells expressing CD27 and CD70 play a key role in regulating B-cell activation and immunoglobulin synthesis. Soluble CD27 (sCD27), the extracellular domain of membrane-bound CD27, can be released after lymphocyte activation by differential splicing of the receptor protein or shedding from the cell surface by metalloproteinases (MMPs). Soluble CD27 has been detected in serum, plasma, and urine samples from healthy individuals, and it has been described elevated sCD27 levels in serum of patients with systemic lupus erythematosus, viral infections, and lymphoid malignancies. The sCD27-pool in cancer patients can be significantly enlarged by immunotherapy. CD27 is recognized as a marker for memory B cells and is considered of diagnostic value in patients who have decreased numbers of switched memory B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD27 (TNFRSF7)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.08 µg/mL recombinant mouse CD27L to 2 µg/mL immobilized recombinant mouse CD27 (TNFRSF7)-Fc. ND50 = 0.3 - 1.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2890861 (BioLegend Cat. No. 942003)
AB_2890861 (BioLegend Cat. No. 942004)

Antigen Details

Structure
Homodimer
Distribution

Resting T and B cells, NK cells, hematopoietic progenitor cells

Function
Induces T-cell co-stimulation and B-cell activation, plasma cell differentiation
Interaction
Activated T and B cells, antigen-presenting cells (APC)
Ligand/Receptor
CD27L (CD70)
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, Plasma cells, T cells, Thymocytes
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Camerini D, et al. 1991. J Immunol. 147:3165-9.
  2. Gravestein LA, et al. 1993. Eur J Immunol. 23:943-50.
  3. Prasad KV, et al. 1997. Proc Natl Acad Sci. 94:6346-51.
  4. Kato K, et al. 2007. Exp Hematol. 35:434-42.
  5. van Montfrans J M, et al. 2012. J Allergy Clin Immunol. 129:787-793.
  6. Goto N, et al. 2012. Leuk Lymphoma. 53:1494-500.
  7. Huang J, et al. 2013. J Immunol. 190:6250-8.
Gene ID
21940 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/16/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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