Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex Antibody

Pricing & Availability
Clone
MTS510 (See other available formats)
Other Names
Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MTS510_LEAF_091907
Mouse TLR4/MD2 transfected cells were stained with Ultra-LEAF™ purified CD284 (clone MTS510) (filled histogram), or Ultra-LEAF™ purified rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.
  • MTS510_LEAF_091907
    Mouse TLR4/MD2 transfected cells were stained with Ultra-LEAF™ purified CD284 (clone MTS510) (filled histogram), or Ultra-LEAF™ purified rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.
  • TLR4_MD2_Mouse-Gut_071212
    FFPE mouse gut was stained with Purified Anti-Mouse TLR4/MD2 Complex (Cat# 117602) or Purified Rat IgG2a, κ at 1:50 (10 µg/mL) using 10 mM Sodium Citrate Buffer pH 6.0 for antigen retrieval and polymer detection system. Cellular localization: Cell Membrane.
    Top: Positive Ctrl: FFPE Mouse Gut, 20X Magnification; Bottom: Negative Ctrl: FFPE Mouse Gut, 20X Magnification
  • xTLR4_MD2_Mouse-Gut-Neg-Ctrl_071212
    FFPE mouse gut was stained with Purified Anti-Mouse TLR4/MD2 Complex (Cat# 117602) or Purified Rat IgG2a, κ at 1:50 (10 µg/mL) using 10 mM Sodium Citrate Buffer pH 6.0 for antigen retrieval and polymer detection system. Cellular localization: Cell Membrane.
    Top: Positive Ctrl: FFPE Mouse Gut, 20X Magnification; Bottom: Negative Ctrl: FFPE Mouse Gut, 20X Magnification
Cat # Size Price Quantity Avail. Save
117617 100 µg £165
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117618 1 mg £413
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Description

Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells and T cells. TLR4 has been recognized as critical for host recognition of bacterial LPS in conjunction with MD2.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ba/F3 cell line transfected with mouse TLR4 /MD2 complex
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IHC - Validated
ICFC, IF, IP, Block - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.8 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.8 In addition to flow cytometric uses, the MTS510 antibody has also been used for immunoprecipitation, immunofluorescence, and blocking of LPS-induced stimulation. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 117607). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 117612) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Akashi S, et al. 2000. J. Immunol. 164:3471. (FC, ICFC, IP, Block)
  2. Kawasaki K, et al. 2003. J. Immunol. 170:413. (FC)
  3. Nalbandian G, et al. 2005. J. Immunol. 175:2666. (FC) PubMed
  4. Kanada S, et al. 2011. Blood 117:2211. PubMed
  5. Laplate P, et al. 2011. J. Biol Chem. 286:42494. PubMed
  6. Khan MA, et al. 2006. Infect. Immun. 74:2522. (IF)
  7. Tanigawa T, et al. 2013. Innate Immun. 19:115. PubMed
  8. Akashi S, et al. 2003. J. Exp. Med. 198:1035.

Antigen Details

Structure
Type I transmembrane protein complex
Distribution

Monocytes, B cells and T cells

Function
Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand/Receptor
Bacterial LPS
Cell Type
B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Frleta D, et al. 2003. J. Leukocyte Biol. 74:1064.

Gene ID
21898 View all products for this Gene ID
UniProt
View information about CD284/MD2 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 3    Revision Date: 02/19/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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