Ultra-LEAF™ Purified anti-mouse TIGIT (Vstm3) Antibody

Pricing & Availability
Clone
4D4/mTIGIT (See other available formats)
Other Names
TIGIT, Vstm3, WUCAM, VSIG9
Isotype
Armenian Hamster IgG
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Product Citations
publications
4D4slashmTIGIT_ULEAF_TIGIT_Antibody_052819.png
ConA-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD3 FITC and TIGIT (Vstm3) (clone 4D4/mTIGIT) Ultra-LEAF™ (left) or Armenian Hamster IgG isotype control (right), followed by anti-Armenian hamster IgG PE.
  • 4D4slashmTIGIT_ULEAF_TIGIT_Antibody_052819.png
    ConA-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD3 FITC and TIGIT (Vstm3) (clone 4D4/mTIGIT) Ultra-LEAF™ (left) or Armenian Hamster IgG isotype control (right), followed by anti-Armenian hamster IgG PE.
Cat # Size Price Quantity Avail. Save
156107 100 µg £161
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156108 1 mg £420
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Description

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set and transmembrane domain-containing protein 3 (Vstm3), is a 26 kD, type I transmembrane protein and member of the CD28 family. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. Its binding partners include CD155 (PVR) and CD112 (PVRL2). TIGIT is a negative regulator of NK and T cell activation. Engagement of TIGIT by dendritic cells results in their differentiation into a tolerogenic phenotype, with an increased secretion of IL-10 and a diminished production of IL-12. Mice deficient for TIGIT are more susceptible to autoimmune disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Hamster
Immunogen
Mouse TIGIT Tetramers
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

It was reported that this clone can block the interaction between mouse TIGIT and CD155.

Application References

(PubMed link indicates BioLegend citation)
  1. Joller N, et al. 2011. J. Immunol. 186:1338.

Antigen Details

Structure
Type I transmembrane protein, 26 kD, member of the CD28 family. Contains 1 Ig-like domain and 1 cytoplasmic ITIM motif.
Distribution

Activated T cells, follicular T helper cells, NK, memory T cells, Tregs.

Function
Negative regulator of T cell activation and proliferation. Induction of tolerogenic dendritic cells.
Ligand/Receptor
CD155 (PVR) and CD112 (PVRL2).
Cell Type
Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References
  1. Levin SD, et al. 2011. Eur. J. Immunol. 41:902.
  2. Yu X, et al. 2009. Nat. Immunol. 10:48.
  3. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. USA 106:17858
Gene ID
100043314 View all products for this Gene ID
UniProt
View information about TIGIT on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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