Ultra-LEAF™ Purified anti-mouse/rat CD62P (P-selectin) Antibody

Pricing & Availability
Clone
RMP-1 (See other available formats)
Regulatory Status
RUO
Other Names
GMP-140, Platelet activation-dependent granule membrane protein (PADGEM)
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
RMP-1_ULEAF_CD62P_Antibody_FC_051315.jpg
Thrombin-activated mouse platelets were stained with Ultra-LEAF purified CD62P (clone RMP-1) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • RMP-1_ULEAF_CD62P_Antibody_FC_051315.jpg
    Thrombin-activated mouse platelets were stained with Ultra-LEAF purified CD62P (clone RMP-1) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
Cat # Size Price Quantity Avail. Save
148307 50 µg £79.00
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148308 500 µg £215.00
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Description

CD62P is a 140 kD type I transmembrane glycoprotein, also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Thrombin activated rat platelets
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
ICC, IHC, WB, IP, ELISA, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence6, immunohistochemistry5, ELISA7, Western blotting4, immunoprecipitation4, and blocking in rats1,2 and mice3.

Application References

(PubMed link indicates BioLegend citation)
  1. Walter UM, et al. 1997. Immunology. 92:290. (Block-rats)
  2. Johnston B, et al. 1997. J. Immunol. 159:4514. (Block-rats)
  3. Hickey MJ, et al. 1998. Circ. Res. 83:1124. (Block-mice)
  4. Walter UM, et al. 1997. Hybridoma. 16:249. (WB, IP)
  5. Alvarez A, et al. 2004. Blood 104:402. (IHC)
  6. Piccio L, et al. 2002. J. Immunol. 168:1940. (IF)
  7. Walter UM, et al. 1997. Hybridoma. 16:249. (ELISA)
Product Citations
  1. Perdomo J, et al. 2019. Nat Commun. 10:1322. PubMed
RRID
AB_2565816 (BioLegend Cat. No. 148307)
AB_2565817 (BioLegend Cat. No. 148308)

Antigen Details

Structure
Type I transmembrane glycoprotein, 140 kD
Distribution

Activated platelets, megakaryocytes, endothelial cells

Function
Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
Ligand/Receptor
CD162 (PSGL-1), CD24 and sialylated Lewis X
Cell Type
Endothelial cells, Megakaryocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. Proc. Natl. Acad. Sci. USA 91:7390.

Gene ID
20344 View all products for this Gene ID 25651 View all products for this Gene ID
UniProt
View information about CD62P on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 01/25/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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