Ultra-LEAF™ Purified anti-mouse PD-1H (VISTA) Antibody

Pricing & Availability
Clone
MH5A (See other available formats)
Other Names
PD-1 homolog, Dies1
Isotype
Armenian Hamster IgG
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Product Citations
publications
MH5A_ULEAF_PD-1H_Antibody_102518
C57BL/6 mouse splenocytes were stained with CD8 APC and purified PD-1H (clone MH5A, left) or Armenian Hamster IgG isotype control (right), followed by PE anti-Armenian Hamster IgG.
  • MH5A_ULEAF_PD-1H_Antibody_102518
    C57BL/6 mouse splenocytes were stained with CD8 APC and purified PD-1H (clone MH5A, left) or Armenian Hamster IgG isotype control (right), followed by PE anti-Armenian Hamster IgG.
Cat # Size Price Quantity Avail. Save
143723 100 µg £84
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143724 1 mg £437
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Description

PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
PD-1H- IgG Fc fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: inhibition of graft vs host disease (GVHD), Western blotting, and immunohistochemical staining of paraffin embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Flies DB, et al. 2011. J. Immunol. 187:1537.
RRID
AB_2783107 (BioLegend Cat. No. 143723)
AB_2783108 (BioLegend Cat. No. 143724)

Antigen Details

Structure
Type I transmembrane protein, member of the CD28 family
Distribution

Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells

Function
Negative regulator of the immune response
Cell Type
Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
Biology Area
Immunology
Antigen References

1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp Med. 208:577.

Gene ID
74048 View all products for this Gene ID
UniProt
View information about PD-1H on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house unless otherwise indicated. However upon request these can be tested from outside independent laboratories on custom basis.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 10/25/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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