Ultra-LEAF™ Purified anti-mouse IFN-γ Recombinant Antibody

Pricing & Availability
Clone
QA20A28 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
QA20A28_ULEAF_IFN-gamma_Antibody_091522
Recombinant mouse IFN-γ (Cat. No. 575302) (black circles) binds to immobilized recombinant mouse IFNGR1 (Fc Tag). Ultra-LEAF™ Purified anti-mouse IFN-γ recombinant (clone QA20A28) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG1, κ isotype control (Cat. No. 400432) (gray triangles) does not have the effect. This antibody blocks the binding of 0.025 µg/mL of recombinant mouse IFN-γ (Cat. No. 575302) to 1 µg/mL immobilized recombinant mouse IFNGR1 (Fc Tag). ND50 range: 0.009 - 0.05 µg/mL.
  • QA20A28_ULEAF_IFN-gamma_Antibody_091522
    Recombinant mouse IFN-γ (Cat. No. 575302) (black circles) binds to immobilized recombinant mouse IFNGR1 (Fc Tag). Ultra-LEAF™ Purified anti-mouse IFN-γ recombinant (clone QA20A28) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG1, κ isotype control (Cat. No. 400432) (gray triangles) does not have the effect. This antibody blocks the binding of 0.025 µg/mL of recombinant mouse IFN-γ (Cat. No. 575302) to 1 µg/mL immobilized recombinant mouse IFNGR1 (Fc Tag). ND50 range: 0.009 - 0.05 µg/mL.
Cat # Size Price Quantity Check Availability Save
606853 100 µg £284
Check Availability


Need larger quantities of this item?
Request Bulk Quote
606854 1 mg £825
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Immunogen
Recombinant mouse IFN-γ
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.025 µg/mL recombinant mouse IFN-γ (Cat. No 575302) to 1 µg/mL immobilized recombinant mouse IFNGR1 (Fc Tag). ND50 range: 0.009 - 0.05 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2927866 (BioLegend Cat. No. 606853)
AB_2927866 (BioLegend Cat. No. 606854)

Antigen Details

Structure
Cytokine; dimer
Distribution

CD8+ and CD4+ T cells, NK cells

Function
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Interaction
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
  2. De Maeyer E, et al. 1992. Curr Opin Immunol. 4:321-6.
  3. Farrar M, et al. 1993. Annu Rev Immunol. 11:571-611.
  4. Gray P, et al. 1987. Lymphokines. 13:151.
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/15/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Ultra-LEAF™ Purified anti-mouse IFN-γ Recombinant Antibody

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account