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Ultra-LEAF™ Purified anti-mouse CNTF Antibody

Pricing & Availability
Clone
W17199A (See other available formats)
Regulatory Status
RUO
Other Names
Ciliary neurotrophic factor
Isotype
Rat IgG2a, λ
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Product Citations
publications
W17199A_ULEAF_CNTF_Antibody_040721
Recombinant mouse CNTF (Cat. No. 761706) (black circles) induces the proliferation of TF-1 cells. Ultra-LEAF™ purified anti-mouse CNTF antibody (clone W17199A) (purple squares) inhibits the effect of recombinant mouse CNTF at 0.2 μg /mL in TF-1 cells in a dose-dependent manner whereas the LEAF™ purified rat IgG2a, λ isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 0.15 - 1 μg/mL.
  • W17199A_ULEAF_CNTF_Antibody_040721
    Recombinant mouse CNTF (Cat. No. 761706) (black circles) induces the proliferation of TF-1 cells. Ultra-LEAF™ purified anti-mouse CNTF antibody (clone W17199A) (purple squares) inhibits the effect of recombinant mouse CNTF at 0.2 μg /mL in TF-1 cells in a dose-dependent manner whereas the LEAF™ purified rat IgG2a, λ isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 0.15 - 1 μg/mL.
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938803 100 µg £185
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938804 1 mg £490
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Description

CNTF, ciliary neurotrophic factor, was initially identified as a survival factor for cultured embryonic chick parasympathetic neurons. The human protein has 84% similarity to the rat and rabbit proteins. It lacks the N-terminal signal sequence and consensus sequences for glycosylation or hydrophobic regions. The CNTF profile shows similar sequence and structure motifs to hematopoietic cytokines (IL-6, IL-11, LIF, oncostatin M, cardiotrophin-1) and thus was considered a member of the IL-6 family. CNTF exerts effects on glial cells and neurons. CNTF activates astrocytes and promotes their capacity to support neurons and oligodendroglia. CNTF stimulates astrocytes to secrete FGF-2 and rat microglia to secrete glial cell line-derived neurotrophic factor (GDNF). CNTF is produced by astrocytes after brain injury. The CNTF receptor complex includes the CNTF binding protein (CNTFRα), the LIF binding protein (LIFRb), and the signal transducer of IL-6 (gp130). CNTFRα does not have transmembrane or intracellular domains, which means it does not induce signal transduction per se. CNTFRα is linked to the cell membrane via glycosyl phosphatidylinositol linkage, and it can be released by phospholipase C to produce a soluble receptor. The complex formed by CNTFRα and CNTF serve as agonists for cells that do not express CNTFRα. Cardiotrophin-like cytokine (CLC) also binds to CNTFRα, which proposed to the activation of gp130, which are LIFRb subunits. CNTF reduces the symptoms of experimental autoimmune encephalomyelitis, and its absence exacerbates the severity of multiple sclerosis disability. CNTF has been studied in retinal degenerative disorders and was shown as a potential clinical application.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CNTF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by the inhibition of proliferation induced by recombinant mouse CNTF (200 ng/mL) on TF-1 cells. ND50 range: 0.15 -1 μg /mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2892501 (BioLegend Cat. No. 938803)
AB_2892501 (BioLegend Cat. No. 938804)

Antigen Details

Structure
Dimer
Distribution

Astrocytes, microglia, hepatocytes, skeletal muscle, embryonic stem cells, and bone marrow stromal cells

Function
Stimulates gene expression, along with cell survival or differentiation in neuronal cell types. It has a potent trophic factor for motor neurons. IL-1b, TNFα (TNF-α), and EGF induces the release of CNTF from astrocytes.
Interaction
Sensory, sympathetic, ciliary, motor neurons, microglia, retinal ganglion cells, adipocytes, and skeletal muscle cells
Ligand/Receptor
Trimeric complex form by CNTF receptor-α (CNTFRα), LIF receptor (LIFR), and gp130.
Antigen References

1. Negro A, et al. 1991. Eur. J. Biochem. 201:289.
2. Bazan JF. 1991. Neuron. 7:197.
3. Davis S, et al. 1993. Science. 260:1805.
4. Plun-Favreau H, et al. 2001. EMBO J. 20:1692.
5. Lin HW, et al. 2009. J. Neuroinflammation 6:7.
6. Wen R, et al. 2012. Prog. Retin. Eye. Res. 31:136.

Gene ID
12803 View all products for this Gene ID
UniProt
View information about CNTF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 04/07/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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