Ultra-LEAF™ Purified anti-mouse CD357 (GITR) Antibody

Pricing & Availability
Clone
DTA-1 (See other available formats)
Other Names
Glucocorticoid-induced TNFR -related gene, TNFRSF18
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
126321 100 µg £67
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126322 1 mg £91
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126323 5 mg £329
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126324 25 mg £860
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126325 50 mg £1,438
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126326 100 mg £2,066
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Description

GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF receptor superfamily, also known as TNFRSF18 and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD25+ CD4+ Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-κB activation, and abrogate the inhibitory function of CD25+ CD4+ Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD25+ CD4+ T cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IP, FA - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. De Luca A, et al. 2007. J. Immunol. 179:5999. PubMed
RRID
AB_2814005 (BioLegend Cat. No. 126321)
AB_2814006 (BioLegend Cat. No. 126322)
AB_2814007 (BioLegend Cat. No. 126323)
AB_2814008 (BioLegend Cat. No. 126324)
AB_2814009 (BioLegend Cat. No. 126325)
AB_2814010 (BioLegend Cat. No. 126326)

Antigen Details

Structure
66-70 kD homodimer of TNFR superfamily member.
Distribution

Low levels on resting T cells, B cells, and macrophages; high levels on CD25+ CD4+ Tregs and CD25+ CD4+ CD8- thymocytes.

Function
Stimulation of GITR can abrogate or reduce the inhibitory function of CD25+ CD4+ Treg cells. In vivo activation of GITR may lead to the development of autoimmune diseases.
Ligand/Receptor
GITR ligand (GITRL).
Cell Type
B cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Shimizu J, et al. 2002 Nat. Immunol. 3:135.
3. Stephens GL, et al. 2004. J. Immunol. 173:5008.
4. McHugh RS, et al. 2002. Immunity 16:311.

Regulation
Activation of T and B cells can upregulate GITR expression.
Gene ID
21936 View all products for this Gene ID 21936 View all products for this Gene ID
UniProt
View information about GITR on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/20/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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