- N2B2 (See other available formats)
- Other Names
- TNFSF10, TNF-related apoptosis inducing ligand, Apo-2 ligand, Apo-2L, CD253
- Rat IgG2a, κ
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- Product Citations
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CD253 is a 40 kD TNF superfamily member known as TRAIL, Apo-2 ligand, and Apo-2L. TRAIL is expressed on a variety of cells, including IL-2 and IL-15 activated NK cells and activated T cells. However, it is undetectable on resting T and B cells. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. The N2B2 antibody has been reported to be useful for flow cytometric staining and blocking NK cell cytotoxicity in vitro.Product Details
- Antibody Type
- Host Species
- Mouse TRAIL-transfected 2PK-3 cells
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Qualit tested
Block - Reported in literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro blocking of NK cell cytotoxicity1,2. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 109315 and 109316).
(PubMed link indicates BioLegend citation)
- Kayagaki N, et al. 1999. J. Immunol. 163:1906. (Block)
- Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
- Joshi PS, et al. 2006. J. Leukocyte Biol. 80:1345.
- Herold S, et al. 2008. J. Exp. Med. 205:3065. PubMed
- Iannello A, et al. 2009. J. Virol. 83:5999. PubMed
- Komatsu M, et al. 2003. Blood 101:3991. (Block)
- Taieb J, et al. 2006. Nature Med. 12:214. (Block)
- Gonzaga R, et al. 2011. J. Immunol. 187:6235. PubMed.
AB_2813976 (BioLegend Cat. No. 109315)
AB_2813977 (BioLegend Cat. No. 109316)
- TNF ligand superfamily member, approximately 40 kD
Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells
- Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity
- TRAIL receptor DR5
- Cell Type
- Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Kayagaki N, et al. 1999. J. Immunol. 163:1906.
2. Wiley SR, et al. 1995. Immunity. 3:673.
3. Wu GS, et al. 1999. Cancer Res. 59:2770.
4. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.
- Gene ID
- 22035 View all products for this Gene ID
- View information about CD253 on UniProt.org
- Does BioLegend test each LEAF™ antibody by functional assay?
No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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