Ultra-LEAF™ Purified anti-mouse CD20 Recombinant Antibody

Pricing & Availability
Clone
QA18A73 (See other available formats)
Regulatory Status
RUO
Other Names
Ms4a1, Ly-44
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
QA18A73_ULEAF_CD20_Antibody_033022
C57BL/6 mouse splenocytes were stained with anti-mouse/human CD45R/B220 APC and purified anti-mouse CD20 recombinant (clone QA18A73) (left) or purified mouse IgG2a, κ isotype control (right), followed by PE anti-mouse IgG2a.
  • QA18A73_ULEAF_CD20_Antibody_033022
    C57BL/6 mouse splenocytes were stained with anti-mouse/human CD45R/B220 APC and purified anti-mouse CD20 recombinant (clone QA18A73) (left) or purified mouse IgG2a, κ isotype control (right), followed by PE anti-mouse IgG2a.
  • QA18A73_ULEAF_CD20_Antibody_2_032922
    C57BL/6 mice were retro-orbitally injected with 200 µg of Ultra-LEAF™ purified anti-mouse CD20 (clone SA271G2, black line, N=4), Ultra-LEAF™ purified anti-mouse CD20 recombinant (clone QA18A73, purple line, N=4), or vehicle only (PBS, red line, N=4). Peripheral blood was analyzed at indicated intervals, and average % B cells calculated for each group (measured as % CD19+B220+ cells).
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161407 100 µg £65
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161408 1 mg £102
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Description

CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Mouse CD20 transfected cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Depletion - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This recombinant antibody clone is derived from clone SA271G2.

Additional Product Notes

A single i.v. dose of 200 µg of mAb clone QA18A73 depletes B cells from peripheral blood for more than 30 days.

RRID
AB_2910341 (BioLegend Cat. No. 161407)
AB_2910341 (BioLegend Cat. No. 161408)

Antigen Details

Structure
Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
Distribution

B cells and a subset of T cells

Function
Regulates B-cell activation and proliferation
Interaction
Associates with MHC class I and II, CD53, CD81, and CD82
Antigen References

1. Morsy DE, et al. 2013. J. Immunol. 191:3112.
2. Pang X, et al. 2017. Hum Gene Ther. 28(2):216-225.
3. Uchida J, et al. 2004. Int Immunol. 16(1):119-29

Gene ID
12482 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/24/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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