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Ultra-LEAF™ Purified anti-mouse CD155 (PVR) Antibody

Pricing & Availability
Clone
A18179E (See other available formats)
Regulatory Status
RUO
Other Names
PVR, PVS, Nectin-like molecule-5, Nectin-like protein 5, NECL5, NECL-5, Poliovirus receptor
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
A18179E_ULEAF_CD155_PVR_Antibody_031521
Biotinylated recombinant mouse TIGIT (black circles) binds to immobilized recombinant mouse CD155-Fc chimera (Cat. No. 786606). Ultra-LEAF™ purified anti-mouse CD155 (PVR) antibody (clone A18179E) (purple squares) inhibits the binding in a dose-dependent manner whereas the rat IgG2a, λ isotype ctrl antibody (gray triangles) does not have the effect. This antibody blocks the binding of 0.2 µg/mL biotinylated recombinant mouse TIGIT to 1 µg/mL immobilized recombinant mouse CD155-Fc chimera. ND50 = 0.05 – 0.25 µg/mL.
  • A18179E_ULEAF_CD155_PVR_Antibody_031521
    Biotinylated recombinant mouse TIGIT (black circles) binds to immobilized recombinant mouse CD155-Fc chimera (Cat. No. 786606). Ultra-LEAF™ purified anti-mouse CD155 (PVR) antibody (clone A18179E) (purple squares) inhibits the binding in a dose-dependent manner whereas the rat IgG2a, λ isotype ctrl antibody (gray triangles) does not have the effect. This antibody blocks the binding of 0.2 µg/mL biotinylated recombinant mouse TIGIT to 1 µg/mL immobilized recombinant mouse CD155-Fc chimera. ND50 = 0.05 – 0.25 µg/mL.
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942103 100 µg £245
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942104 1 mg £715
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Description

CD155, also known as TAGE4 or NECL-5 (nectin-like molecule-5), was originally described as a poliovirus entry receptor (PVR) more than 30 years ago. CD155 is a 70 kD type I transmembrane glycoprotein that belongs to the nectin-related family of adhesion molecules within the immunoglobulin superfamily, defined by the presence of immunoglobulin domains V, a C1-like domain and a C2 domain in the extracellular region. CD155 binds several molecules including vitronectin, nectin-3, DNAM-1/CD226, CD96, and TIGIT. All bindings are mediated by the V-type domain and alternative splicing within this domain can modulate ligand binding. CD155 is highly expressed on tumor cells of epithelial and neuronal origin. Its enhanced expression in tumors contributes to the loss of contact inhibition leading to increased migration, however, tumor cell recognition and killing by NK cells are also enhanced via DNAM-1 or CD96 expressing NK cells. CD155 normally functions in the establishment of intercellular adherent junctions between epithelial cells. CD155 mediates NK cell adhesion and triggers NK cell effector functions by binding to CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD155
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Blocking - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.2 µg/mL biotinylated recombinant mouse TIGIT to 1 µg/mL immobilized recombinant mouse CD155-Fc chimera. ND50 = 0.05 – 0.25 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2890862 (BioLegend Cat. No. 942103)
AB_2890862 (BioLegend Cat. No. 942104)

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

Highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin, preferentially expressed on Th17 cells.

Function
Mediates NK cell adhesion and triggers NK cell effector functions
Interaction
NK cell, T cell
Ligand/Receptor
Vitronectin, nectin-3, DNAM-1/CD226, CD96, and TIGIT
Cell Type
Dendritic cells, Th17, Thymocytes
Biology Area
Adaptive Immunity, Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Neuroscience
Molecular Family
Adhesion Molecules, Soluble Receptors
Antigen References
1. He Y, et al. 2000. Proc. Natl. Acad. Sci. USA. 97:79-84. 
2. Bottino C, et al. 2003. J. Exp. Med. 198:557-67. 
3. Fuchs A, et al. 2004. J. Immun. 172: 3994-3998. 
4. Reymond N, et al. 2004. J. Exp. Med. 199:1331-41. 
5. Koike S, et al. 1990. EMBO J. 9: 3217-3224. 
6. Mendelsohn CL, et al. 1989. Cell 56:855-65.
Gene ID
52118 View all products for this Gene ID
UniProt
View information about CD155 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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