Ultra-LEAF™ Purified anti-mouse CD134 (OX-40) Antibody

Pricing & Availability
Clone
OX-86 (See other available formats)
Other Names
TNFRSF4, ACT35, OX-40
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
OX-86_LEAF_CD134_Antibody_020818
Con A-stimulated (3 days) C57BL/6 splenocytes were stained with CD3 APC and CD134 (OX-40) (clone OX-86) LEAF™ Purified (left) or rat IgG1, κ LEAF™ Purified isotype control (right), followed by anti-rat IgG1 PE.
  • OX-86_LEAF_CD134_Antibody_020818
    Con A-stimulated (3 days) C57BL/6 splenocytes were stained with CD3 APC and CD134 (OX-40) (clone OX-86) LEAF™ Purified (left) or rat IgG1, κ LEAF™ Purified isotype control (right), followed by anti-rat IgG1 PE.
Cat # Size Price Quantity Avail. Save
119429 100 µg £67
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119430 1 mg £91
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119431 5 mg £329
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119432 25 mg £860
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119433 50 mg £1,438
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119434 100 mg £2,066
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Description

CD134 is a type I integral membrane protein also known as OX-40, ACT35, and tumor necrosis factor receptor superfamily member 4 (TNFRSF4). This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX-40 receptor binds to the OX-40 ligand (CD252) to provide a costimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, which implies that these interactions are important in the pathogenesis of some autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse OX-40-CD4 chimeric protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone OX-86 has been reported to act as an agonist and stimulate OX-40.

Application References

(PubMed link indicates BioLegend citation)
  1. Higgins LM, et al. 1999. J. Immunol. 162:486. (FC, IHC)
  2. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695. (Costim)
  3. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed

Antigen Details

Structure
TNF receptor superfamily, 50 kD
Distribution

Activated CD4+ and CD8T cells, activated B cells.

Function
Receptor for OX-40 ligand, provides co-stimulatory signal for lymphocyte proliferation independent of CD28; thought to play a role in the pathogenesis of some autoimmune diseases
Ligand/Receptor
OX-40 ligand
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695.
2. Weinberg AD, et al. 1999. J. Immunol. 162:1818.
3. Akira H, et al. 1999. J. Immunol. 162:7058.
4. Pippig SD, et al. 1999. J. Immunol. 163:6520.
5. Higgins LM, et al. 1999. J. Immunol. 162:486.

Gene ID
22163 View all products for this Gene ID
UniProt
View information about CD134 (OX-40) on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/13/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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