Ultra-LEAF™ Purified anti-mouse CD120b (TNF R Type II/p75) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

E. coli -expressed mouse Type II TNFR

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block, IP, ELISA Capture, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204).
ELISA Capture¹: The TR75-54.7 antibody is useful as a capture antibody when paired with the biotinylated TR75-32.4 antibody (Cat. No. 113203/113204) as the detecting antibody.
Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting¹, and receptor blocking¹. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 113305-113310).

Application References

(PubMed link indicates BioLegend citation)

1. Sheehan KC, et al. 1995. J. Exp. Med. 181:607.

RRID

AB_2832383 (BioLegend Cat. No. 113305)
AB_2832384 (BioLegend Cat. No. 113306)
AB_2832385 (BioLegend Cat. No. 113307)
AB_2832386 (BioLegend Cat. No. 113308)
AB_2876426 (BioLegend Cat. No. 113309)
AB_2832387 (BioLegend Cat. No. 113310)

Antigen Details

Structure

TNFR superfamily, 75 kD

Distribution

Variety of cell types at low levels

Function

Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation

Ligand/Receptor

TNF-α, LT-α (TNF-β)

Cell Type

Tregs

Biology Area

Immunology, Innate Immunity

Molecular Family

CD Molecules, Cytokine/Chemokine Receptors

Antigen References

1. Aggarwal BB, et al. 1985. Nature 318 665.
2. Chan FKM, et al. 2000. Science 288:2351.
3. Loetscher H, et al. 1990. Cell 61:351.
4. Rothe J, et al. 1993. Nature 364:798.

Gene ID

21938 View all products for this Gene ID

UniProt

View information about CD120b on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis