Ultra-LEAF™ Purified anti-human IL-22 Antibody

Pricing & Availability
Clone
A16057A (See other available formats)
Other Names
TIFa, IL-D110, IL-TIF, TIFIL-23, zcyto18
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A16057A_ULEAF_IL-22_Antibody_1_082219
Recombinant human IL-22 (Black circles, Cat. No. 571306) stimulates the production of IL-10 in human Colo 205 cells. Anti-human IL-22 antibody (clone A16057A, purple squares) inhibits the effect of recombinant human IL-22 at 0.75 ng/mL in human Colo 205 cells in a dose-dependent manner. The ND50 = 1.2 - 6 μg/mL.
  • A16057A_ULEAF_IL-22_Antibody_1_082219
    Recombinant human IL-22 (Black circles, Cat. No. 571306) stimulates the production of IL-10 in human Colo 205 cells. Anti-human IL-22 antibody (clone A16057A, purple squares) inhibits the effect of recombinant human IL-22 at 0.75 ng/mL in human Colo 205 cells in a dose-dependent manner. The ND50 = 1.2 - 6 μg/mL.
Cat # Size Price Quantity Avail. Save
618303 100 µg £165
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618304 1 mg £437
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Description

IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29. Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human IL-22
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by the neutralization of IL-10 production induced by human recombinant IL-22 (0.75 ng/mL) on human Colo 205 cells. ND50 range: 1.2 - 6.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814466 (BioLegend Cat. No. 618303)
AB_2814467 (BioLegend Cat. No. 618304)

Antigen Details

Structure
Cytokine
Distribution

Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells

Function
Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; Promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis
Interaction
Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
Ligand/Receptor
IL-22R1 and IL-10R2 receptor chains
Cell Type
Epithelial cells, NK cells, T cells, Th17
Biology Area
Cell Biology, Immunology, Neuroinflammation, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Nagalakshmi ML, et al. 2004. Intern Immunopharmacol. 4:679-691.
  2. Kebir H, et al. 2007. Nat Med. 13:1173-1175.
  3. Gu Y, et al. 2008. Eur J Immunol. 38:1807-1813.
  4. Pene J, et al. 2008. J Immunol. 180:7423-7430.
  5. Dumotier L, et al. 2000. J immune 164:1814-1819.
  6. Xie MH, et al. 2000. J Biol Chem. 765:31335-31339.
  7. Jones BC, et al. 2008. Structure. 16:1333-44
  8. Kotenko SV, et al. 2001. J Immunol. 166:7096-71033.
  9. Chang C, et al. 2006. Cell Res 16:902-907.
Gene ID
50616 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08/22/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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