Ultra-LEAF™ Purified anti-human CNTF Antibody

Pricing & Availability
Clone
A15088B (See other available formats)
Other Names
Ciliary Neurotrophic Factor, HCNTF
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15088B_ULEAF_CNTF_Antibody_121418
Recombinant human CNTF (grey triangles, Cat. No. 559802) induces the proliferation of TF-1 human erythroleukemic cells. Anti-human CNTF antibody (clone A15088B, purple squares) inhibits the effect of recombinant human CNTF (at 400 ng/mL) in TF-1 cells in a dose dependent manner. The ND50 = 0.2 – 1.0 µg/mL.
  • A15088B_ULEAF_CNTF_Antibody_121418
    Recombinant human CNTF (grey triangles, Cat. No. 559802) induces the proliferation of TF-1 human erythroleukemic cells. Anti-human CNTF antibody (clone A15088B, purple squares) inhibits the effect of recombinant human CNTF (at 400 ng/mL) in TF-1 cells in a dose dependent manner. The ND50 = 0.2 – 1.0 µg/mL.
Cat # Size Price Quantity Avail. Save
608503 100 µg £165
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608504 1 mg £437
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Description

CNTF, ciliary neurotrophic factor, was initially identified as a survival factor for cultured embryonic chick parasympathetic neurons. The human protein has 84% similarity to the rat and rabbit proteins. It lacks an N-terminal signal sequence and consensus sequences for glycosylation or hydrophobic regions. The CNTF profile shows similar sequence and structure motifs to hematopoietic cytokines (IL-6, IL-11, LIF, oncostatin M, cardiotrophin-1) and thus was considered a member of the IL-6 family. CNTF exerts effects on glial cells and neurons. CNTF activates astrocytes, which promote their capacity to support neurons and oligodendroglia. CNTF stimulates astrocytes to secrete FGF-2 and rat microglia to secrete glial cell line-derived neurotrophic factor (GDNF). CNTF is produced by astrocytes after brain injury. The CNTF receptor complex includes the CNTF binding protein (CNTFRα), the LIF binding protein (LIFRb), and the signal transducer of IL-6 (gp130). CNTFRα does not have transmembrane or intracellular domains, which means it does not induce signal transduction per se. CNTFRα is linked to the cell membrane via glycosyl phosphatidylinositol linkage, and it can be released by phospholipase C to produce a soluble receptor. The complex formed by CNTFRα and CNTF serve as agonists for cells that do not express CNTFRα. Cardiotrophin-like cytokine (CLC) also binds to CNTFRα, which is proposed to activate gp130, which are LIFRb subunits. CNTF reduces the symptoms of experimental autoimmune encephalomyelitis, and its absence exacerbates the severity of multiple sclerosis disability. CNTF has been studied in retinal degenerative disorders and was shown as a potential clinical application.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CNTF
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The anti-human CNTF antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF solution contains no preservative; handle under aseptic conditions. Do not freeze.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the inhibition of proliferation induced by human recombinant CNTF (400 ng/mL) on TF‑1 human erythroleukemic cells. ND50 range: 0.2 – 1.0 mg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783353 (BioLegend Cat. No. 608503)
AB_2783354 (BioLegend Cat. No. 608504)

Antigen Details

Structure
Homodimer
Distribution

Astrocytes, microglia, hepatocytes, skeletal muscle, embryonic stem cells, and bone marrow stromal cells.

Function
Stimulates gene expression, along with cell survival or differentiation in neuronal cell types. It is a potent trophic factor for motor neurons. IL-1b, TNFα (TNF-α), and EGF induces the release of CNTF from astrocytes.
Interaction
Sensory, sympathethic, ciliary, motor neurons, microglia, retinal ganglion cells, adipocytes, and skeletal muscle cells.
Ligand Receptor
Trimeric complex form by CNTF receptor-α(CNTFRα), LIF receptor (LIFR), and gp130/ CD130.
Cell Type
Astrocytes, Embryonic Stem Cells, Microglia
Biology Area
Cell Biology, Stem Cells
Antigen References
  1. Negro A, et al. 1991. Eur. J. Biochem. 201:289.
  2. Bazan JF. 1991. Neuron 7:197.
  3. Davis S, et al. 1993. Science 260:1805.
  4. Plun-Favreau H, et al. 2001. EMBO J. 20:1692.
  5. Lin HW, et al. 2009. J Neuroinflammation 6:7.
  6. Wen R, et al. 2012. Prog. Retin. Eye Res. 31:136.
Gene ID
1270 View all products for this Gene ID
UniProt
View information about CNTF on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house unless otherwise indicated. However upon request these can be tested from outside independent laboratories on custom basis.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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