Ultra-LEAF™ Purified anti-human CD80 Antibody

Pricing & Availability
Clone
W17149G (See other available formats)
Other Names
B-Lymphocyte activation antigen B7.1, CD80, Ly53, TSA1, CD28 ligand (CD28L), MIC17
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17149G_ULEAF_CD80_Antibody_101019.png
Recombinant biotinylated hCTLA-4 (black circles) binds to immobilized recombinant hCD80. Anti-hCD80 antibody (clone W17149G, purple squares) inhibits the binding in a dose-dependent manner whereas the GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl Antibody (Cat. No. 400666, green triangles) does not have the effect. This antibody blocks the binding of 10 ng/mL recombinant biotinylated hCTLA-4 to 1 µg/mL immobilized recombinant hCD80 with an ND50 of 0.05-0.4 µg/mL.
  • W17149G_ULEAF_CD80_Antibody_101019.png
    Recombinant biotinylated hCTLA-4 (black circles) binds to immobilized recombinant hCD80. Anti-hCD80 antibody (clone W17149G, purple squares) inhibits the binding in a dose-dependent manner whereas the GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl Antibody (Cat. No. 400666, green triangles) does not have the effect. This antibody blocks the binding of 10 ng/mL recombinant biotinylated hCTLA-4 to 1 µg/mL immobilized recombinant hCD80 with an ND50 of 0.05-0.4 µg/mL.
  • W17149G_ULEAF_CD80_Antibody_2_101019.png
    Recombinant biotinylated hCD80 (black circles) binds to immobilized recombinant hCD28. Both anti-hCD80 antibody (clone W17149G, purple squares) and GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl Antibody (Cat. No. 400666, green triangles) do not inhibit the binding between hCD80 and hCD28.
Cat # Size Price Quantity Avail. Save
621803 100 µg £165
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621804 1 mg £437
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Description

CD80, also known as B7-1, is a member of the B7 family of proteins. The B7 family of proteins are structurally related, cell membrane-associated molecules that regulate immune responses by delivering costimulatory or coinhibitory signals via their ligands. So far, eight family members have been identified, including B7-1, B7-2, B7-H1, B7-H2, B7-H4, B7-H6, PD-L1, and PD-L2. These proteins are mainly expressed by activated antigen presenting cells (APCs) and when paired with either CD28 or CTLA-4 on a T lymphocyte, they induce an increase or decrease in the downstream signaling activity of the MHC-TCR interaction between APCs and T cells. B7-1 has been shown to bind both CTLA-4 and CD28; however, it has a 20-fold higher affinity to CTLA-4 than to CD28. Despite their structural similarities, the individual contribution of B7-1 and B7-2 to the development of pathogenic T cells in autoimmune diseases and protective T cells in infectious diseases is markedly distinct. Besides regulating T cell immune responses, B7 also plays a role in anti-tumor immunity. This is confirmed by demostrating that cytotoxic T cells were able to eradicate tumor cells transfected with B7-1 and B7-2. After activation, follicular lymphoma was shown to upregulate B7-1 and other costimulatory and adhesion molecules, therefore increasing APC activity and amplifying primed T cell responses. Homozygous null B7-1/B7-2 mice fail to generate antigen specific IgG1 and IgG2a responses, suggesting that B7 proteins are important in Ig immune responses. Mouse and human B7-1 share 44% amino acid identity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD80 Recombinant Protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between hCD80 and hCTLA-4. The ND50 range is 0.05 – 0.4 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Homodimer
Distribution

Activated B cells, monocytes, macrophages, dendritic cells

Function
Plays a role in T cell activation; CD80 and CD86 play an important role in T cell priming.
Interaction
T cells
Ligand/Receptor
CD28, CTLA-4
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Freeman GJ, et al. 1993. Science. 262:909.
  2. Azuma M, et al. 1993. Nature. 366:76.
  3. Gimmi CD, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:6575.
  4. Linsley PS, et al. 1991 J. Exp. Med. 173:721.
  5. Greaves P and Gribben JG, 2013. Blood. 121: 734.
  6. Czuczman MS, et al. 2005. J. Clin. Oncol. 23:4390.
Gene ID
941 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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