Ultra-LEAF™ Purified anti-human CD40 Antibody

Pricing & Availability
Clone
W17212H (See other available formats)
Other Names
Tumor necrosis factor receptor superfamily member 5, TNFRSF5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
W17212H_ULEAF_CD40_Antibody_1_083019_updated
Recombinant biotinylated hCD40L (Black circles) binds to immobilized recombinant hCD40 (Cat. No. 777206). Anti-hCD40 antibody (clone W17212H, Purple squares) inhibits the binding in a dose-dependent manner whereas the Isotype control (Cat. No. 400666, Green triangles) doesn’t have the effect. This antibody blocks the binding of 80 ng/mL hCD40L biotinylated protein to 1 µg/mL immobilized recombinant hCD40 with an ND50 = 0.2 - 1 µg/mL.
  • W17212H_ULEAF_CD40_Antibody_1_083019_updated
    Recombinant biotinylated hCD40L (Black circles) binds to immobilized recombinant hCD40 (Cat. No. 777206). Anti-hCD40 antibody (clone W17212H, Purple squares) inhibits the binding in a dose-dependent manner whereas the Isotype control (Cat. No. 400666, Green triangles) doesn’t have the effect. This antibody blocks the binding of 80 ng/mL hCD40L biotinylated protein to 1 µg/mL immobilized recombinant hCD40 with an ND50 = 0.2 - 1 µg/mL.
Cat # Size Price Quantity Avail. Save
668103 100 µg £165
Check Availability


Need larger quantities of this item?
Request Bulk Quote
668104 1 mg £437
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD40, also known as TNFRSF5, is a type I transmembrane protein that belongs to the TNF receptor family (TNFR). CD40 is expressed on B cells, dendritic cells, monocytes, macrophages, platelets, basal epithelial cells, endothelial cells and a subset of CD34+ hematopoietic progenitors. The interaction between CD40 and its ligand TNFSF5/CD40L leads to the clustering of CD40 and the recruitment of TNFR associated factors (TRAFs), including TRAF1, 2, 3, 5 and 6. TRAF1, 2, 5 and 6 serve as positive regulators while TRAF3 is a negative regulator. CD40/CD40L interaction triggers downstream signaling on both cells, including upregulation of CD40 and cell survival on dendritic cells, upregulation of co-stimulatory molecules, CD80 and CD86 on T cells, and production of inflammatory cytokines. In addition, CD40 regulates B cell development/maturation, Ig isotype switching and in combination with other signals such as IL-4, promotes proliferation. It has been reported that a transcriptional factor, AKNA, can coordinately regulate the expression of CD40 and CD40L. Dysregulation of CD40 is associated with several diseases, such as hyper-IgM immunodeficiency type 3 (HIGM3) and Hodgkin's disease. Targeting of CD40/CD40L for cancer therapy is being evaluated in clinical trials, including anti-CD40 agonist antibodies and soluble recombinant CD40L. Several isoforms have been identified in humans and mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD40 Recombinant Protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between hCD40 and hCD40L. The ND50 range: 0.2 - 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814510 (BioLegend Cat. No. 668103)
AB_2814511 (BioLegend Cat. No. 668104)

Antigen Details

Structure
Disulfide bond-linked homodimer
Distribution

B cells, dendritic cells, monocytes, macrophages, platelets, basal epithelial cells, endothelial cells and a subset of CD34+ hematopoietic progenitors, primary carcinomas.

Function
CD40 regulates B cell development/maturation, Ig isotype switching proliferation. CD40 is also involved in T cell priming and inflammatory cytokine production.
Interaction
TRAF1, 2, 3, 5, 6
Ligand/Receptor
TNFSF5/CD40L
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, Platelets
Biology Area
Cancer Biomarkers, Immuno-Oncology, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Ridge JP, et al. 1998. Nature 393:474.
  2. Schoenberger SP, et al. 1998. Nature 393:480.
  3. Bremer E. 2013. ISRN Oncol. 2013:371854.
  4. Siddiqa A, et al. 2001. Nature. 410: 383.
  5. Durandy A, et al. 2005. Immunol Rev. 203:67.
  6. O'Grady JT, et al. 1994. Am J Pathol. 144:21.
  7. Karaca NE, et al. 2012. Scand J Immunol. 76:21.
  8. Tone M, et al. 2001. Proc Natl Acad Sci USA. 98:1751.
  9. Eshel D, et al. 2008. Mol Immunol. 46:250.
  10. Hostager BS, et al. 2000. J Biol Chem. 275:15392.
  11. Elgueta R, et al. 2009. Immunol Rev. 229:152.
Gene ID
958 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08/23/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account