Ultra-LEAF™ Purified anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
A17188B (See other available formats)
Other Names
PD1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17188B_ULEAF_CD279_Antibody_100719.png
Recombinant hPD-1 binds to hPD-L1. Anti-human PD-1 Antibody (clone A17188B, purple squares) inhibits the binding in a dose-dependent manner. The ND50 =0.1 – 0.5 µg/mL.
  • A17188B_ULEAF_CD279_Antibody_100719.png
    Recombinant hPD-1 binds to hPD-L1. Anti-human PD-1 Antibody (clone A17188B, purple squares) inhibits the binding in a dose-dependent manner. The ND50 =0.1 – 0.5 µg/mL.
Cat # Size Price Quantity Avail. Save
621603 100 µg £165
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621604 1 mg £437
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Description

Programmed cell death protein 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD279 protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking hPD-1 and hPD-L1 binding in the prescence of 0.8 µg/mL hPD-1 Biotin. ND50 range: 0.1– 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

A17188B antibody can block the binding of NAT105 and EH12.2H7 antibodies to the target.

Antigen Details

Structure
55 kD type I transmembrane protein
Distribution

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.

Function
Signaling and co-stimulation (co-inhibition)
Interaction
SHP-1 and SHP-2
Ligand/Receptor
PD-L1 (CD274) and PD-L2 (CD273)
Cell Type
Lymphocytes, T cells
Biology Area
Cell Biology, Immuno-Oncology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References
  1. Ishida Y, et al. 1992. EMBO J. 11:3887
  2. Francisco LM, et al. 2010. Immunol Rev. 236:219
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 10/07/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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